Reconstruction of ligament using mesenchymal stem cells

使用间充质干细胞重建韧带

• 批准号: 13671489
• 负责人: TAKAGISHI Kenji
• 金额: $ 2.37万
• 依托单位: Gunma University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2001
• 资助国家: 日本
• 起止时间: 2001 至 2003
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-13671489/
• 关键词: Mesenchymal stem cells; 靭帯; 靱帯; 骨髄; 幹細胞; 軟骨

Mesenchymal stem cells harvested from the bone marrow are cultured in vitro and differentiated into the objected cells to repair the tissue defect. However, ligament tissue is hard to make. In this study, we tried to make the ligament tissue using stem cells. Stem cells were harvested from bone marrow in the rabbit and proliferated and embedded in the type I collagen gel. Stem cells secreted the type I collagen and present same character as the cells in the ligament. Cells differentiated into the cartilage cells showed the type II collagen.

从骨髓中获取的骨髓间充质干细胞在体外培养并分化为目标细胞，用于修复组织缺损。然而，韧带组织很难制作。在本研究中，我们尝试用干细胞制作韧带组织。从兔骨髓中获取干细胞，并将其增殖并包埋在I型胶原凝胶中。干细胞分泌I型胶原，呈现出与韧带细胞相同的特征。分化为软骨细胞的细胞呈II型胶原。

项目成果

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