Immunity to type II collagen and proteoglycan in OA.

OA 中对 II 型胶原蛋白和蛋白多糖的免疫。

• 批准号: 05671229
• 负责人: TAKAGISHI Kenji
• 金额: $ 1.28万
• 依托单位: Kitasato University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1993
• 资助国家: 日本
• 起止时间: 1993 至 1995
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-05671229/
• 关键词: Osteoarthritis; Type II collagen; Proteoglycan; Mice; Immunity; Human; Rheumatoid arthritis; 免疫反応; プロテオグリカン

We measured immunity to type II collagenand proteoglycans in osteoarthritic C57BL mice to determine whether it is related to osteoarthritis pathogenesis. Histological examination revealed articular cartilage lesions in all mice and synovitis in only a few mice. Immunological responses to type IIcollagen were found in collagen arthritic mice, but not in C57BL mice. Furthermore, immunological responses to proteoglycans were observed in proteoglycanimmunized mice, but not in C57BL mice Therefore, articular cartilage degeneration may not result in autoimmunity to type II collagen and proteoglycans in osteoarthrits of C57BL mice, and immune responses to these We also measured the serum anti-type II collagen antibody in osteoarthritic patients. No humoral immunity was revealed in healthy people and definete anti-type II collagen antibody was showed in several patients with rheumatoid arthritis. In one of fifty osteoarthritic patients, the humoral immunity to type II collagen was revealed. In human osteoarthritis. Immunity to type II collagen might be related to the pathogenesis of the disease in a certain patient.

我们检测了骨关节炎C57 BL小鼠对II型胶原和蛋白多糖的免疫，以确定其是否与骨关节炎发病机制有关。组织学检查显示所有小鼠关节软骨病变，仅少数小鼠出现滑膜炎。胶原性关节炎小鼠对II型胶原有免疫应答，而C57BL小鼠无免疫应答。此外，在蛋白多糖免疫的小鼠中观察到对蛋白多糖的免疫应答，而在C57BL小鼠中未观察到。因此，关节软骨退变可能不导致对C57BL小鼠骨关节炎中的II型胶原和蛋白多糖的自身免疫，并且对这些免疫应答。健康人无体液免疫反应，部分类风湿关节炎患者有明确的抗Ⅱ型胶原抗体。在50例骨关节炎患者中，发现了对II型胶原的体液免疫。在人类骨关节炎中。对Ⅱ型胶原的免疫反应可能与本病的发病有关。

项目成果

K.Takagishi,K.Maeda: "Absence of immunity to type II collagen and proteoglpan in asteoarthritis C57BL mice" Revista Espanola de Rheumatologia. 317 (1993)

K.Takagishi、K.Maeda：“骨关节炎 C57BL 小鼠对 II 型胶原蛋白和蛋白多糖缺乏免疫力”Revista Espanola de Rheumatologia。

Takagishi K.: "Absence of Immunity to type II collagen and proteoglycan in osteoarthritic C57 Black mice" Inflammation Research. 44. 222-226 (1995)

Takagishi K.：“骨关节炎 C57 黑小鼠对 II 型胶原蛋白和蛋白多糖缺乏免疫力”炎症研究。

Takagishi K., Maeda K., Goso Y., Itoman M,Yamamoto M.: "Absence of immunity tu type II collagen and proteoglycan in osteoarthritic C57BL mice." Inflammation Research. 44. 222-226 (1995)

Takagishi K.、Maeda K.、Goso Y.、Itoman M、Yamamoto M.：“骨关节炎 C57BL 小鼠缺乏 II 型胶原蛋白和蛋白多糖的免疫力。”

Takagishi K., Itoman M,Goso Y., Kuwao S., Miyahara H., Kaibara N.: "Effects of cyclosporin on serum hyaluronan levels in collagen arthritis." Inflammation Research. 44. 79-82 (1995)

Takagishi K.、Itoman M、Goso Y.、Kuwao S.、Miyahara H.、Kaibara N.：“环孢素对胶原关节炎血清透明质酸水平的影响。”

Takagishi K.et al: "Absence of immunity to type II colagen and proteoglycan in osteoarthritic C57BLmice" Inflammation Research. 44. 222-226 (1995)

Takagishi K.等人：“骨关节炎 C57BL 小鼠对 II 型胶原和蛋白多糖缺乏免疫力”炎症研究。