Acceleration of angiogenesis in necrotic bone by combining vascular bundle implantation and FGF-2 administration

本研究以24只日本大白兔为研究对象，通过血管束植入和FGF-2联合给药促进坏死骨血管生成。取一部分兔髂骨作为游离骨移植物，在液氮中冷冻以确保细胞完全坏死。在骨头上开一个窄洞，移植物被放置在大腿近端。第1组，在骨孔内单次注射100微克FGF-2，使大隐动脉及其伴静脉穿过骨孔。第二组以孔内注射生理盐水，同时植入维管束作为对照。在手术后第1周和第2周观察血管周围的新生血管形成情况。从第1周到第2周，两组血管密度逐渐增加。术后1周，1组血管密度（0.148）显著高于2组（0.050）。术后2周，1组血管密度平均值为0.375,2组血管密度平均值为0。194. 这一差异也具有统计学意义。术后1周，1组血管长度（0.862mm）明显高于2组（0.330mm）。术后2周，1组血管长度平均值为2.625mm， 2组血管长度平均值为1.383mm。在本研究中，局部单次注射FGF-2可改善坏死骨的手术血管生成。由于FGF-2被认为是多种间充质细胞的有效丝裂原，因此血管束植入和FGF-2联合施用可能有助于治疗骨缺血性坏死

The aim of this study is to accelerate the angiogenesis in necrotic bone by combining vascular bundle implantation and FGF-2 administrationTwenty-four Japanese white rabbits were used. A portion of rabbit iliac crest bone was removed as a free bone graft and frozen in liquid nitrogen to ensure complete cellular necrosis. A narrow hole was created in the bone and the graft was placed in the proximal thigh. In group 1, FGF-2 was injected into the hole at a single dose of 100 micrograms, and the saphenous artery and its venae comitantes were passed through the hole of the bone. In group 2, injection of saline into the hole and vascular bundle implantation was used as a control. Neovascularization around the vessel was evaluated at weeks 1 and 2 after vestmentNeovascularization was observed along the implanted vascular bundle. The vessel density gradually increased from week 1 to week 2 in both groups. At 1 week after surgery, the vessel density in group 1 (0.148) was significantly higher than in group 2 (0.050). At 2 weeks after surgery, the mean of the vessel density in group 1 was 0.375 and that in group 2 was 0. 194. This difference was also statistically significant. The length of vessels at 1 week after surgery in group 1 (0.862mm) was significantly higher than in group 2 (0.330mm). At 2 weeks after surgery, the mean of the vessel length in group 1 was 2.625mm and that in group 2 was 1.383mm. This difference was also statistically significantA local single injection of FGF-2 improved surgical angiogenesis in necrotic bone in this study. As FGF-2 is recognized as a potent mitogen for a variety of mesenchymal cells, the combination of vascular bundle implantation and FGF-2 administration may contribute to the treatment of avascular necrosis of bone