Acceleration of angiogenesis in necrotic bone by combining vascular bundle implantation and FGF-2 administration

结合维管束植入和 FGF-2 给药加速坏死骨中的血管生成

• 批准号: 13671511
• 负责人: ISHIDA Osamu
• 金额: $ 0.96万
• 依托单位: HIROSHIMA UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2001
• 资助国家: 日本
• 起止时间: 2001 至 2002
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-13671511/
• 关键词: FGF-2; osteonecrosis; angiogenesis; treatment; growth factor

The aim of this study is to accelerate the angiogenesis in necrotic bone by combining vascular bundle implantation and FGF-2 administrationTwenty-four Japanese white rabbits were used. A portion of rabbit iliac crest bone was removed as a free bone graft and frozen in liquid nitrogen to ensure complete cellular necrosis. A narrow hole was created in the bone and the graft was placed in the proximal thigh. In group 1, FGF-2 was injected into the hole at a single dose of 100 micrograms, and the saphenous artery and its venae comitantes were passed through the hole of the bone. In group 2, injection of saline into the hole and vascular bundle implantation was used as a control. Neovascularization around the vessel was evaluated at weeks 1 and 2 after vestmentNeovascularization was observed along the implanted vascular bundle. The vessel density gradually increased from week 1 to week 2 in both groups. At 1 week after surgery, the vessel density in group 1 (0.148) was significantly higher than in group 2 (0.050). At 2 weeks after surgery, the mean of the vessel density in group 1 was 0.375 and that in group 2 was 0. 194. This difference was also statistically significant. The length of vessels at 1 week after surgery in group 1 (0.862mm) was significantly higher than in group 2 (0.330mm). At 2 weeks after surgery, the mean of the vessel length in group 1 was 2.625mm and that in group 2 was 1.383mm. This difference was also statistically significantA local single injection of FGF-2 improved surgical angiogenesis in necrotic bone in this study. As FGF-2 is recognized as a potent mitogen for a variety of mesenchymal cells, the combination of vascular bundle implantation and FGF-2 administration may contribute to the treatment of avascular necrosis of bone

本研究旨在通过血管束植入和FGF-2联合应用促进坏死骨的血管生成。取出一部分兔髂嵴骨作为游离骨移植物，并在液氮中冷冻，以确保细胞完全坏死。在骨中创建一个窄孔，并将移植物放置在大腿近端。第1组，将FGF-2以100 μ g的单次剂量注入骨孔中，并将隐动脉及其伴行静脉穿过骨孔。第2组为对照组，孔内注射生理盐水，植入血管束。术后1周和2周观察血管周围新生血管形成情况。两组血管密度从第1周到第2周逐渐增加。术后1周，第1组的血管密度（0.148）显著高于第2组（0.050）。术后2周，第1组的平均血管密度为0.375，第2组为0。194.该差异也具有统计学显著性。术后1周，第1组血管长度（0.862 mm）显著高于第2组（0.330 mm）。术后2周，第1组血管长度平均值为2.625 mm，第2组为1.383 mm。在本研究中，局部单次注射FGF-2可改善坏死骨的手术血管生成。由于FGF-2被认为是多种间充质细胞的有效有丝分裂原，因此血管束植入和FGF-2给药的组合可能有助于治疗骨缺血性坏死

项目成果

Atsuo Nakamae: "Acceleration of surgical angiogenesis in necrotic bone with a single injection of fibroblast growth factor-2 (FGF-2)"Journal of orthopaedic research. (in press).

Atsuo Nakamae：“单次注射成纤维细胞生长因子 2 (FGF-2) 加速坏死骨中的手术血管生成”骨科研究杂志。