Allogeneic Leg Transplantation With A New Method For Bone Marrow Transplantation

同种异体腿部移植采用骨髓移植新方法

• 批准号: 13671549
• 负责人: NAKAMURA Masaya
• 金额: $ 1.34万
• 依托单位: Kansai Medical University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2001
• 资助国家: 日本
• 起止时间: 2001 至 2003
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-13671549/
• 关键词: IBM-BMT; leg-transplantation; fludarabine phosphate; 下肢移植; トレランス; Fludarabine phosphate

We have recently established a new method for bone marrow transplantation(BMT) in mice : bone marrow cells(BMCs) are directly injected into the intra-bone marrow(IBM) cavity(IBM-BMT). In the present study, we carried out allogeneic leg transplantation in rats in conjunction with IBM-BMT. Brown Norway(BN: RTIA^n) rats were intravenously injected with 50mg/kg of fludarabine phosphate, followed by sublethal fractionated irradiation(4.5Gy×2) one day before IBM-BMT. The hind limb from a Fischer344(F344:RTIA^1) rat was transplanted on day 0, and BMCs(3×10^7 cells/100μ1) obtained from the donor F344 rat were injected into the bone marrow cavity of the left tibia of the recipient BN rat. The hematolymphoid cells in the recipient BN rats were completely reconstituted by the cells of the donor F344 rat. The limbs transplanted from the donor F344 rats were accepted for >1yr without any clinical signs of rejection. The lymphocytes of the BN rats showed tolerance to both donor-type and recipient-type major histocompatibility complex(MHC) determinants in mixed lymphocyte reaction, but showed a significant response to the third party MHC determinations. Using a combination of the injection of fludarabine, low-dose irradiation, and IBM-BMT, we have succeeded in allogeneic limb transplantation without using any immunosuppressants after the operation.

我们最近建立了一种新的小鼠骨髓移植方法：骨髓细胞直接注入骨髓腔内（IBM-BMT）。在本研究中，我们进行了异基因大鼠小腿移植与IBM-BMT。布朗挪威（BN：RTIA（n）大鼠在IBM-BMT前1d静脉注射磷酸氟达拉滨50 mg/kg，然后进行亚致死剂量分割照射（4.5Gy×2）。在第0天移植Fischer 344（F344：RTIA ^1）大鼠的后肢，并将从供体F344大鼠获得的BMC（3×10^7个细胞/100μ l）注射到受体BN大鼠的左胫骨的骨髓腔中。受体BN大鼠的血淋巴细胞完全被供体F344大鼠的细胞重建。供者F344大鼠的肢体移植接受超过1年，无任何排斥反应的临床表现。BN大鼠的淋巴细胞在混合淋巴细胞反应中对供者型和受者型主要组织相容性复合体（MHC）决定簇均表现出耐受性，但对第三方MHC决定簇表现出显著的反应性。采用氟达拉滨注射液、低剂量照射和IBM-BMT的联合应用，我们成功地进行了同种异体肢体移植，术后未使用任何免疫抑制剂。

项目成果

Takashi Esumi: "Successful Allogeneic Leg Transplantation In Rats In Conjunction With Intra-Bone Marrow Injection Of Donor Bone Marrow Cells"TRANSPLANTATION. Vol.76 No.11. 1543-1548 (2003)

Takashi Esumi：“在大鼠中成功进行同种异体腿部移植并结合骨髓内注射供体骨髓细胞”移植。

Takashi Esumi: "Successful allogeneic leg transplantation in rats in conjunction with intra-bone marrow injection of donor bone marrow cells"TRMSPLANTATION. Vol76No11. 1543-1548 (2003)

Takashi Esumi：“在大鼠中成功进行同种异体腿移植，并结合骨髓内注射供体骨髓细胞”TRMSPLANTATION。

Takashi Esumi: "SUCCESSFUL ALLOGENEIC LEG TRANSPLANTATION IN RATS IN CONJUNCTION WITH INTRA-BONE MARROW INJECTION OF DONOR BONE MARROW CELLS"Transplantation. Vol.76,No.11. 1543-1548 (2003)

Takashi Esumi：“在大鼠中成功进行同种异体腿部移植并结合供体骨髓细胞的骨髓内注射”移植。