Transplantation of iPS cell derived neural stem cells for spinal cord injury

iPS细胞衍生的神经干细胞移植治疗脊髓损伤

• 批准号: 21390426
• 负责人: NAKAMURA Masaya
• 金额: $ 10.73万
• 依托单位: Keio University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2009
• 资助国家: 日本
• 起止时间: 2009 至 2011
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-21390426/
• 关键词: 脊髄損傷; 人工多能性幹細胞; 移植; 再生医療; 細胞移植; コモンマーモセット

In the present study, we demonstrated that transplantation of mouse induced pluripotent stem cells(iPSC) derived neural stem/progenitor cells(NS/PCs) promoted functional recovery after spinal cord injury(SCI) in mice. Furthermore, transplantation of human iPSC-NS/PC also promoted functional recovery after SCI in NOD/scid mice as well as common marmosets without any tumor formation as long as pre-evaluated''safe" human iPSC-NS/PCs were used as a cell source. Pre-evaluated'safe' human iPSC-NS/PCs s may be a promising cell source for transplantation therapy for patients with SCI.

在本研究中，我们证明了小鼠诱导多能干细胞（iPSC）衍生的神经干/祖细胞（NS/PC）移植促进小鼠脊髓损伤（SCI）后的功能恢复。此外，人iPSC-NS/PC的移植也促进了NOD/scid小鼠以及普通绒猴SCI后的功能恢复，而没有任何肿瘤形成，只要使用预先评估的“安全”人iPSC-NS/PC作为细胞来源。预先评估的“安全”的人iPSC-NS/PC可能是SCI患者移植治疗的有希望的细胞来源。

项目成果

Evaluation of human fetal neural stem/progenitor cells as cell sources for cell replacement therapy for neurological disorders : their property change after long in vitro maintenance and long-term tumorigenicity in vivo

评估人胎儿神经干/祖细胞作为神经系统疾病细胞替代疗法的细胞来源：长期体外维持和体内长期致瘤性后其特性变化

• 发表时间: 2009
• 期刊: J Neurosci Res 87
• 作者: Ogawa D;Okada Y;Nakamura M;Shimazaki T;et al.
• 通讯作者: et al.

Pre-evaluated safe human iPS clone derived neural stem cells promoted functional recovery after spinal cord injury without tumorigenicity in adult common marmosets

预先评估的安全人类 iPS 克隆衍生的神经干细胞可促进成年普通狨猴脊髓损伤后的功能恢复，且无致瘤性

• 发表时间: 2011
• 作者: Kobayashi Y;Okada Y;Iwai H;Nishimura S;Nori S;Konomi T;Fujiyoshi K;Tsuji O;Toyama Y;Yamanaka S;Okano H;Nakamura M
• 通讯作者: Nakamura M

Transplantation of human iPS cell-derived neurospheres for the treatment of spinal cord injury in NOD-scid mice. GCOE幹細胞医学のための教育拠点成果報告シンポジウム

人类 iPS 细胞来源的神经球移植治疗 NOD-scid 小鼠脊髓损伤 GCOE 干细胞医学教育中心结果研讨会。

• 发表时间: 2010
• 作者: Nori S;Okada Y;Tsuji O;Takahashi Y;Fujiyoshi K;Yasuda A;Toyama Y;Yamanaka S;Nakamura M;Okano H
• 通讯作者: Okano H

Physiological signinficance of astrogliosis after CNS injury

中枢神经系统损伤后星形胶质细胞增生的生理意义

• 发表时间: 2009
• 期刊: Inflammationand Regeneration. 29
• 作者: Okada S;Nakamra M;Saiwai H;et al.
• 通讯作者: et al.

Transplantation of human iPS cell-derived neurospheres into injured spinal cord of NOD-scid mice

将人类 iPS 细胞衍生的神经球移植到 NOD-scid 小鼠受损的脊髓中

• 发表时间: 2009
• 作者: 海苔聡;岡田洋平;中村雅也
• 通讯作者: 中村雅也