Strategy for spinal cord injury-induced pain

脊髓损伤引起的疼痛的策略

基本信息

批准号：
13671599
负责人：
KAWAMATA Mikito
金额：
$ 2.5万
依托单位：
Sapporo Medical University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2001
资助国家：
日本
起止时间：
2001 至 2002
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-13671599/
关键词：
spinal cord injury hyperalgesia spinal dorsal norn wide-dynamic-range neuron high-threshold neuron

项目摘要

Background : Spinal cord injury(SCI) results in chronic central neuropathic pain in dermatomes caudal to the lesion(below-level pain) and rostral to the lesion(above-level pain), which has remained refractory to clinical, treatment despite the development of various therapeutic strategies. In order to gain a better understanding of the pain, we investigated changes in physiological and pharmacological properties of spinal dorsal horn neurons in spinal segments rostral and caudal to the lesion in rats with SCI.Methods : The right spinal cord of Sprague-Dawley rats was hemisected at the level of L2. At 10 to 14 days after the SCI, when mechanical hyperalgesia/allodynia had fully developed, spontaneous activity and evoked responses to non-noxious and noxious mechanical stimuli of wide-dynamic-range(WDR) and high-threshold(HT) neurons rostral and caudal to the lesion were recorded. Cumulative doses of morphine were administered systemically(0.1-3mg/kg) and spinally(0.1-5μg), and spontaneou … More s activity and evoked responses to the stimuli of the neurons were evaluated.Results : Spontaneous activity significantly increased in WDR neurons both rostral and caudal to the SCI site, but high-frequency background discharges with burst patterns were observed only in neurons rostral to the SCI site. After the SCI, which involved cutting the L2 dorsal root and hemisectioning the spinal cord, WDR and HT neurons rostral to the SCI site still had receptive fields on the skin of the hindpaw, dermatomes L3,L4 and L5. Significant increases in responses to the mechanical stimuli were seen both in WDR and HT neurons located both rostrally and caudally to the lesion. The responses to non-noxious and noxious stimuli were significantly greater in WDR neurons caudal to the SCI site than those in WDR neurons rostral to the SCI site. In contrast, the responses to noxious stimuli were significantly higher in HT neurons rostral to the SCI site than those in HT neurons caudal to the SCI site. Systemically administered morphine had a greater effect on responses to non-noxious and noxious stimuli of WDR neurons rostral to the SCI site than those caudal to the SCI site. Spinally administered morphine significantly suppressed responses of WDR neurons in SCI animals to non-noxious stimuli compared to those in sham-operated control animals.Conclusions : The findings suggest that changes in properties of spinal dorsal horn neurons after SCI are caused by different physiological and pharmacological mechanisms depending on the classification of the neurons and their segmental locations and that different spinal mechanisms contribute to SCI-induced pain. Less

背景：脊髓损伤（SCI）会导致皮肤皮肤的慢性中枢神经性疼痛（尾部疼痛）（低于水平的疼痛）和病变（高于水平的疼痛），尽管有各种治疗策略的发展，但仍对临床进行了治疗。为了更好地了解疼痛，我们调查了脊柱脊髓脊髓神经元的物理和药物特性的变化，在SCI.Methods的大鼠中，脊髓和尾部的病变：Sprague-Dawley大鼠的右脊髓在L2的水平下半分。 SCI后10至14天，当机械性痛觉过敏/合金充分发育时，赞助活动并引起对宽动力范围（WDR）（WDR）的非毒性和有害的机械刺激的反应，并记录了the剂（HT）Neurons rostral和CAUDAL对房间的记录。评估了吗啡的累积剂量（0.1-3mg/kg）和Spontaneou…评估了对神经元刺激的更多活性和诱发反应。回应：WDR神经元的自发性显着增加了SCI部位的WDR神经元，但与SCI部位的NEURENS相比，SIC的距离是造成的。在SCI涉及切割L2背根并将脊髓进行半分析的SCI之后，WDR和HT神经元的SCI部位仍然具有后爪，皮肤皮肤L3，L4和L5的皮肤上的接收场。在位于病变的WDR和HT神经元中，对机械刺激的反应显着增加。 WDR神经元对SCI部位的对非氧化和有害刺激的反应明显大于SCI位于SCI位点的SCI部位。相比之下，HT神经元对SCI部位的有害刺激的反应明显高于SCI部位的HT神经元尾部的反应。与SCI部位相比，系统给予的吗啡对WDR神经元对SCI部位的无毒理和有害刺激的反应更大。与假手术对照动物相比，SCI动物中WDR神经元对非毒性刺激的反应的反应显着抑制的反应显着抑制的反应，与假手术对照动物的反应显着抑制。结论表明，SCI后SCI脊髓背角神经元的性质的变化是由不同的物理和药物分类的分类引起的，SCI及其在SCI后的分类引起的，这表明，SCI的特性的变化是不同的，这表明SCI动物的刺激及其分类不同疼痛。