Investigation into the cross-talk of cell adhesion molecule in the brain on the mechanism, of neuronal protection

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We had been investigating the effect of plasma fihronectin(pFn) to focal cerebral ischemia using the conditional knockout mouse which is pFn deficient until 2001, We found that pFn supports neuronal survival and reduces brain injury following transient focal cerebral ischemia with anti-apoptotic effect which were recognized by the measurement of TUNEL and caspaseS and so oa We have started the study which is the effect of cyclosporinA(CsA) to focal cerebral ischemia on mice, leading to the research of pFn anti-apoptotic effect. CsA, however, is impermeable by blood-brain barrier(BBB), and we used mdrla knockout mice which don't have the active efflux of CsA from brain to blood. We found that CsA demonstrated the anti-ischenaic effect on the mice following following transient focal cerebral ischemia. On the contrary, CsA also showed the neurotoxic effect, depending on dosage of CsA Additionally, the infarct volume of mdrla knockout mice without CsA-treated waa significantly bigger than that of wild-type mice, suggesting that mdrla may be effective to brain injury after transient ischemia.