The Jackson Laboratory Knockout Mouse Production and Phenotyping Project (JAX KOMP2)

项目摘要 Knokout Mouse表型项目(KOMP)的目标是提供一个全面的目录 哺乳动物通过创建8,500个基因敲除和执行 系统化、高通量的表型流水线。2011年秋季推出，三个生产/表型 包括日本宇宙航空研究开发机构在内的中心得到资助，并被分配到模型创建和广泛的表型鉴定任务。 第一阶段成功地产生了2500个品系并进行了表型鉴定。杰克逊第二阶段的目标 实验室(JAX)KOMP计划(KOMP2)在当前资助期(8/1/16-7/31/21)将创建 1000个小鼠基因敲除(KO)系，并使用 高效、宽广的检测流水线。2020年3月11日，世界卫生组织(WHO)宣布 严重急性呼吸综合征冠状病毒2型(新冠肺炎)在全球范围内暴发 大流行。大流行的一个后果是我们现有的人员和服务减少 需要达到我们最初的目标。这项资金延期请求的目的是超越目前的 项目期是为了确保成熟的模型生产管道的连续性，并提供 不间断供应用于表型鉴定的小鼠品系。我们要求延长该项目的资金，以便 12个月，新的项目期结束日期为2022年7月31日。

Project Summary The goal of the Knockout Mouse Phenotyping Project (KOMP) is to provide a comprehensive catalog of mammalian gene function obtained through the creation of 8,500 gene knockouts and execution of a systematic, high-throughput phenotyping pipeline. Launched in the fall of 2011, three production/phenotyping centers including JAX were funded and assigned the task of model creation and broad-based phenotyping. The first phase succeeded in generating and phenotyping 2,500 lines. The goals of phase 2 of The Jackson Laboratory (JAX) KOMP program (KOMP2) during the current funding period (8/1/16 – 7/31/21) are to create 1000 knockout (KO) lines of mice and perform high-throughput phenotyping across organ systems using an efficient and broad testing pipeline. On March 11, 2020 the World Health Organization (WHO) declared the outbreak of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) disease (COVID-19) a world-wide pandemic. A consequence of the pandemic has been a reduction of our available personnel and services needed to reach our original goals. The purpose of this request for a funded extension beyond the current project period is to ensure continuity of well-developed model production pipelines, and to provide an uninterrupted supply of mouse lines for phenotyping. We are requesting the project be extended with funds for 12 months, with a new project period end date of 07/31/2022.