The Jackson Laboratory Knockout Mouse Production and Phenotyping Project (JAX KOMP2)

项目摘要 敲除小鼠表型项目（KOMP）的目标是提供一个全面的目录， 通过创建8，500个基因敲除和执行一个 系统化、高通量表型分析管道。2011年秋季推出，三个生产/表型 包括JAX在内的研究中心都获得了资助，并被分配了创建模型和进行广泛的表型分析的任务。 第一阶段成功地产生了2,500个品系并进行了表型分析。杰克逊第二阶段的目标 实验室（JAX）KOMP计划（KOMP 2）在当前资助期内（2016年8月1日至2021年7月31日）将创建 1000个敲除（KO）小鼠系，并使用 高效和广泛的测试管道。2020年3月11日，世界卫生组织宣布， 严重急性呼吸系统综合征冠状病毒2型（SARS-CoV-2）疾病（COVID-19）在全球范围内爆发 流行病这一流行病的一个后果是我们现有的人员和服务减少 我们需要达到最初的目标。这一要求的目的是在现有的 项目期间，确保开发良好的模型生产管道的连续性，并提供 不间断地提供用于表型分析的小鼠品系。我们要求延长该项目的资金， 12个月，新的项目期结束日期为2022年7月31日。

Project Summary The goal of the Knockout Mouse Phenotyping Project (KOMP) is to provide a comprehensive catalog of mammalian gene function obtained through the creation of 8,500 gene knockouts and execution of a systematic, high-throughput phenotyping pipeline. Launched in the fall of 2011, three production/phenotyping centers including JAX were funded and assigned the task of model creation and broad-based phenotyping. The first phase succeeded in generating and phenotyping 2,500 lines. The goals of phase 2 of The Jackson Laboratory (JAX) KOMP program (KOMP2) during the current funding period (8/1/16 – 7/31/21) are to create 1000 knockout (KO) lines of mice and perform high-throughput phenotyping across organ systems using an efficient and broad testing pipeline. On March 11, 2020 the World Health Organization (WHO) declared the outbreak of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) disease (COVID-19) a world-wide pandemic. A consequence of the pandemic has been a reduction of our available personnel and services needed to reach our original goals. The purpose of this request for a funded extension beyond the current project period is to ensure continuity of well-developed model production pipelines, and to provide an uninterrupted supply of mouse lines for phenotyping. We are requesting the project be extended with funds for 12 months, with a new project period end date of 07/31/2022.