The relationship of the inflammatory infiltrating cell in prostate grand and chemokine

（1）前列腺炎性细胞浸润的病理学研究1998年至2000年在我所因浸润性膀胱癌行根治性膀胱切除术的患者纳入本研究。浸润细胞类型通过使用一系列抗体CD 20、CD 45 RO、CD 68、S-100的免疫染色来评估。浸润细胞类型以T细胞为主。32.4%的病例可见炎性细胞浸润。（2）抗微生物药物对前列腺癌细胞株PC-3细胞因子分泌的抑制作用。上清液的IL-8浓度根据人型支原体的浓度而增加。RT-PCR检测PC-3细胞中Toll样受体-2和Toll样受体-4的mRNA表达。NF-κB活性随支原体浓度的增加而增加。这些结果表明Toll样受体信号可上调NF-κB B活性，导致IL-8的产生。RT-PCR检测GFLX对IL-8 mRNA表达的影响。GFLX可显著降低TNF-α诱导的IL-8 mRNA水平。GFLX以剂量依赖性方式抑制PC-3分泌IL-8。结果提示，GFLX对慢性前列腺炎有一定的抗炎作用。

(1)The pathological study of inflammatory cell infiltration in the prostate in patientsPatients entered into the study received radical cystoprostatectomy because of invasive bladder cancer in our institute from 1998 to 2000. The infiltrating cell type was assessed by immunostaining using a series of antibodies CD2O, CD45RO, CD68,S-100. The types of infiltrating cells were dominantly T cells. Inflammatory cell infiltration was seen in 32.4% cases.(2)Antimicrobial agents and suppression of cytokine secretion of prostate cancer cell line.PC-3,a human prostate cancer cell lines, was used for the experiment. IL-8 concentrations of the supematants were increased depending on the concentrations of Mycoplasma hominis. The mRNA of Toll like receptor-2 and Toll like receptor4 were demonstrated by RT-PCR in PC-3 cell lines. The activities of NF-κB were increased depending on the concentration of Mycoplasma hominisa. These data show that the signals from Toll-like receptor up the activities of NF-κB and lead to produce of IL-8. The influence of GFLX on IL-8 mRNA expression was analyzed using RT-PCR. GFLX significantly attenuated the TNF-α induced IL-8 mRNA level. GFLX suppresses the secretion of IL-8 from PC-3 in a dose-dependent manner. The results may imply that GFLX has an anti-inflammatory effect on chronic prostatitis.