Clinical and genetic analysis of the causes of male infertility

由于这种疾病的各种原因，男性不育患者的治疗方式从未建立起来。本研究试图从临床、遗传学和流行病学的角度阐明男性不育的原因。在精液分析中，当精子计数和精子活力正常时，患者被诊断为正常。然而，有人怀疑，如果他们的伴侣在妇科检查中没有发现任何问题，他们中的一些人可能有精子功能异常，包括顶体反应。采用计算机辅助精子分析仪(CASA)对精子数和精子活动率正常的男性不育患者和正常生育男性志愿者进行精子运动质量检测。不幸的是，两组之间的精子运动功能没有显著差异。与CASA一样，使用PENETRAK和顶珠试验，不育和生育男子之间的精子功能没有显著差异。另一方面，对于S…通过原位末端标记法和BrdU表达检测，进一步探讨不育症患者生精功能低下的原因、细胞凋亡和细胞增殖的可能性。基于这些结果，精子生成不足的原因并不统一，其中一些原因表现为精原细胞更新减少，原因是其凋亡率增加。作为一项流行病学研究，对札幌地区正常年轻健康男性的精子计数和精子活力进行了检测。与过去20年相比，这些数值没有明显变化。有孩子的男性和整个群体之间没有显著差异。男性不育症的增加并不明显。从这些结果可以得出结论，作为男性不育的原因，生精不足的问题比精子功能更关键。最近，精子功能有恶化的迹象，然而，在流行病学上没有发现精子数量减少的证据。较少

Therapeutic modalities for male infertile patients have never been established because of various causes of this disease. In this study, we tried to clarify the causes of male infertility by clinical, genetic and epidemiological approaches.When normal findings of sperm counts and sperm motility were obtained in seminalysis, patients were diagnosed normal. However, it has been suspected that some of them might have functional abnormalities of their sperm including acrosomal reaction, if their partners did not show any problem in gynecological examinations. Quality of sperm motility was testing by CASA (Computed Assisted Sperm Analyzer) in male infertility with normal sperm counts and sperm motility and fertile male volunteers. Unfortunately, no significant difference in sperm motile function was recognized between two groups. As well as CASA, using PENETRAK and acrobeads test, no significant difference in sperm function was shown between infertile and fertile men.On the other hand, to s … More tudy the causes of hypospermatogenesis in patients with infertility, potentiality of apoptosis and cell proliferation was immunohistochemically evaluated using TUNEL method and the detection of the expression of BrdU, respectively. Based on these results, the causes of hypospermatogenesis was not uniform, some of them showed the decrease of spermatogonial renewal due to the increase of its apoptosis.As an epidemiological study, sperm count and sperm motility were examined in normal young healthy males in the Sapporo area. These values were not significantly changed compared to those of the past 20 years. There was no significant difference between men who have a child and a whole group. It was not obvious that male infertility was increasing.From these results, it was concluded that issues of hypospermatogenesis would be more crucial than sperm function as a cause of male infertility. Recently, deterioration of sperm function was suggested, however, no evidence of the decrease of sperm counts was epidemiologically detected. Less