Clinical and genetic analysis of the causes of male infertility

男性不育症患者的治疗方式从未确定，因为这种疾病的原因多种多样。在这项研究中，我们试图从临床，遗传学和流行病学的方法来阐明男性不育的原因。当精原分析中发现精子数量和精子活力正常时，患者被诊断为正常。然而，有人怀疑，如果他们的伴侣在妇科检查中没有出现任何问题，他们中的一些人可能有精子功能异常，包括顶体反应。用CASA（计算机辅助精子分析仪）对精子数量和精子活力正常的男性不育和有生育能力的男性志愿者进行精子活力质量检测。不幸的是，两组之间的精子运动功能没有显著差异。与CASA一样，使用PENETRAK和acrobeads测试，不育和可育男性的精子功能没有显着差异。另一方面，为了进一步研究不育患者精子发生不足的原因，分别采用TUNEL法和检测BrdU表达的免疫组织化学方法评估细胞凋亡和细胞增殖的可能性。综上所述，低精子发生的原因并不统一，有的表现为由于细胞凋亡增加而导致精原细胞更新减少。作为一项流行病学研究，对札幌地区正常年轻健康男性的精子数量和精子活力进行了检查。这些数值与过去20年相比没有显著变化。有孩子的男性和整个群体之间没有显著差异。男性不育增加的趋势不明显。从这些结果可以得出结论，作为男性不育的原因，精子发育不足的问题比精子功能更重要。最近，精子功能的退化被认为，然而，没有证据表明精子数量减少的流行病学检测。少

Therapeutic modalities for male infertile patients have never been established because of various causes of this disease. In this study, we tried to clarify the causes of male infertility by clinical, genetic and epidemiological approaches.When normal findings of sperm counts and sperm motility were obtained in seminalysis, patients were diagnosed normal. However, it has been suspected that some of them might have functional abnormalities of their sperm including acrosomal reaction, if their partners did not show any problem in gynecological examinations. Quality of sperm motility was testing by CASA (Computed Assisted Sperm Analyzer) in male infertility with normal sperm counts and sperm motility and fertile male volunteers. Unfortunately, no significant difference in sperm motile function was recognized between two groups. As well as CASA, using PENETRAK and acrobeads test, no significant difference in sperm function was shown between infertile and fertile men.On the other hand, to s … More tudy the causes of hypospermatogenesis in patients with infertility, potentiality of apoptosis and cell proliferation was immunohistochemically evaluated using TUNEL method and the detection of the expression of BrdU, respectively. Based on these results, the causes of hypospermatogenesis was not uniform, some of them showed the decrease of spermatogonial renewal due to the increase of its apoptosis.As an epidemiological study, sperm count and sperm motility were examined in normal young healthy males in the Sapporo area. These values were not significantly changed compared to those of the past 20 years. There was no significant difference between men who have a child and a whole group. It was not obvious that male infertility was increasing.From these results, it was concluded that issues of hypospermatogenesis would be more crucial than sperm function as a cause of male infertility. Recently, deterioration of sperm function was suggested, however, no evidence of the decrease of sperm counts was epidemiologically detected. Less