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Search for new target antigens of cancer by the SEREX method using cancer patient's serum containing antibodies

利用癌症患者血清中含有的抗体，通过SEREX方法寻找癌症的新靶抗原

基本信息

批准号：
13671680
负责人：
YOSHIKAWA Kazuhiro
金额：
$ 2.3万
依托单位：
AICHI MEDICAL UNIVERSITY
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2001
资助国家：
日本
起止时间：
2001 至 2002
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-13671680/
关键词：
Urological tumor SEREX

项目摘要

The SEREX method is a system to identify unique antibodies and antigens for cancer patients using autologouse cancer tissue and serum and many researchers had found many unique antigens. These results showed that many of these antigens detected expressed in Testis. So, these antigens were named Cancer testis (CT) antigen. These antigens are useful targets to induce cellular immunity to treat cancers, since, on the Spermatogenic cells surface, HLA-class I antigen is not expressed.In this study, we collected cancer tissues and autologouse serums of a prostate and 5 renal cancer, and serums of 3 prostate and 10 renal cancers. We tried to make an expression libraries using mRNAs from prostate cancer tissues and we found that the titer of these libraries were 2.5x10^6 pfu.And we made an expression library using one tissue of two testis obtained. We tested the usefulness of this testis library using cancer serum, which was confirmed to contained some antibodies reacted to cancer tissues, and could get many positive cloned. We determined nucleotide sequences of cDNA fragments in positive clones and found that we got 7 unique clones and many positive clones were same clones. Now, we can't open the name of unique genes which contained come antigens detected in auto-immune disease.These results showed that the our original libraries were useful tools to detect antigens recognized with cancer patients serum. So, we continue to screen the library using several cancer serums.

SEREX方法是一种利用自体肿瘤组织和血清鉴定肿瘤患者特异性抗体和抗原的系统，许多研究者已经发现了许多特异性抗原。这些结果表明，检测到的许多抗原在睾丸中表达。因此，这些抗原被命名为癌症睾丸（CT）抗原。在本研究中，我们收集了1例前列腺癌和5例肾癌的癌组织和自体血清，以及3例前列腺癌和10例肾癌的血清。我们尝试用前列腺癌组织中的mRNA构建表达文库，发现这些文库的滴度为2.5 × 10^6 pfu。我们用癌血清对该文库进行了验证，证实该文库中含有与癌组织反应的抗体，并能获得许多阳性克隆。对阳性克隆中的cDNA片段进行核苷酸序列测定，发现有7个独特的克隆，其中许多阳性克隆是相同的克隆。目前，我们还不能公开含有自身免疫性疾病中检测到的抗原的独特基因的名称，这些结果表明，我们的原始文库是检测癌症患者血清识别抗原的有用工具。因此，我们继续使用几种癌症血清筛选文库。