Molecular Bidogical Analysis of Prostaglandin Synthesis in The Pregnant Myometrium and Fetal Membrane

妊娠子宫肌层和胎膜中前列腺素合成的分子双向分析

• 批准号: 13671707
• 负责人: ITOH Hiroaki
• 金额: $ 2.62万
• 依托单位: KYOTO UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2001
• 资助国家: 日本
• 起止时间: 2001 至 2002
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-13671707/
• 关键词: pregnancy; myometrium; prostaglandin; estrogen; stretch; fetal membrane

The pregnant myometrium relaxes and does not contract in spit of rapidly growing fetus. However, the myometrium contracts vigorously after onset of labor and gives birth to the neonate. Thus moyoetrium is hypothesized to be "quiescent phase" during pregnancy, which changes into "active phase" after initiation of parturition. The aim of the present study is too investigate such dynamic changes of myometrial tonus during pregnancyWe revealed that the expression of COX-2 and/or cPLA_2,the enzymes contributing to prostaglandin synthesis, is locally up-regulated in the decidua and amnion close to dilated cervical canal in labor. In vitro studies suggested that cyclic distension by labor may contribute to such local up-regulation of COX-2 at least partlyOn the other hand, pregnancy significantly elevated COX-1 and prostacyclin synthase (PGIS) expression in the human myometrium. In vitro studies revealed that estrogen significantly elevated both COX-1 and PGIS protein and mRNA expression and that cyclic mechanical stretch significantly augmented PGIS mRNA expression in the transcription levelTherefore, both estrogen and cyclic stretch play important roles in the regulation of prostaglandins production both in fetal membrane as well as myometrim during pregnancy

怀孕的子宫肌层松弛，在快速生长的胎儿中不会收缩。然而，分娩开始后子宫肌层剧烈收缩，产下新生儿。因此，Moyoetrium被假设为“静止期”在怀孕期间，这变成“活跃期”后开始分娩。本研究的目的是探讨妊娠期子宫肌张力的动态变化。我们发现，在分娩时扩张的宫颈管附近的蜕膜和羊膜中，促进前列腺素合成的酶考克斯-2和/或cPLA_2的表达局部上调。体外研究表明，分娩引起的周期性扩张可能至少部分地促进了考克斯-2的局部上调，另一方面，妊娠显著升高了人子宫肌层中考克斯-1和前列环素合酶（PGIS）的表达。体外研究表明，雌激素显著提高考克斯-1和PGIS的蛋白和mRNA表达，周期性机械牵张显著提高PGIS mRNA在转录水平的表达。因此，雌激素和周期性牵张在调节妊娠期子宫内膜和子宫肌层中前列腺素的产生中起重要作用。

项目成果

Masahiro Yoshida, et al.: "Cytokines, prostaglandin F_<2a> and mechanical stretch augment matrix metalloproteinase-1 secretion from cultured human uterine cervical fibroblast cells"Mol Hum Reprod. 8. 681-687 (2002)

Masahiro Yoshida等人：“细胞因子、前列腺素F_2a和机械拉伸增强了培养的人子宫宫颈成纤维细胞的基质金属蛋白酶-1分泌”Mol Hum Reprod。

M Yoshida, H Itoh, 他8名: "Nitric oxide increases matrix metalloproteinase-1 profuction in human uterine cervical fibroblast cells."Mol Hum Reprod. 7. 979-985 (2001)

M Yoshida、H Itoh 和其他 8 人：“一氧化氮增加人子宫宫颈成纤维细胞中基质金属蛋白酶-1 的产量。”Mol Hum Reprod. 7. 979-985 (2001)

Norimasa Sagawa, et al.: "Role of leptin in pregnancy-a review"Placenta. 23. S80-S86 (2002)

Norimasa Sakawa 等人：“瘦素在妊娠中的作用——综述”胎盘。

K Terakawa, H Itoh, 他5名: "Site-specific Augmentation of Amnion Cyclooxygenase-2 and Decidua Vera Phospholipase-A_2 Expression in Labor. Possible Contribution of Mechanical Stretch and Interleukin-1 to Amnion Prostaglandin Synthesis"J Soc Gynelcol Inv. 9.

K Terakawa、H Itoh 和其他 5 人：“分娩中羊膜环氧化酶 2 和蜕膜磷脂酶 A_2 表达的位点特异性增强。机械拉伸和白细胞介素 1 对羊膜前列腺素合成的可能贡献”J Soc Gynelcol Inv. 9。 。

JTelfer, H Itoh, 他7名: "Adivity and Expression of Soluble and Particulate Guanylate Cyclases in Myometrium from Nonpregnant and Pregnant Women : Down-Regulation of Soluble Guanylate Cvclase at Term"J Glin Endoctinol Metab. 86. 5934-5943 (2001)

JTelfer、H Itoh 和其他 7 人：“非妊娠和孕妇子宫肌层中可溶性鸟苷酸环化酶的活性和表达：足月可溶性鸟苷酸环化酶的下调”J Glin Endoctinol Metab 86. 5934-5943 (2001)