Role of leptin and interleukin-8 in human ovary

瘦素和白细胞介素8在人卵巢中的作用

• 批准号: 13671716
• 负责人: IRIE Takashi
• 金额: $ 2.24万
• 依托单位: Tottori University School of Medicine
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2001
• 资助国家: 日本
• 起止时间: 2001 至 2003
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-13671716/
• 关键词: leptin; estrogen; granulosa cell; KGN cells; IL-8; アポトーシス

We evaluated the expression, regulation, and role of leptin and interleukin (IL-8) in human ovary. Follicular fluid and granulosa lutein cells (GLCs) were collected during IVF cycles. KGN cells, the human granulosa cell tumor cell line, were also used. The levels of leptin and IL-8 in follicular fluid and IL-8 protein production were determined using ELISA. The levels of leptin in follicular fluid showed a positive correlation with those of IL-8. Reverse transcription polymerase chain reaction showed the presence of leptin receptor mRNA and IL-8 mRNA in GLCs and KGN cells. In contrast, IL-8 receptor mRNA was not detected in these cells. The addition of E2 and FSH significantly increased the IL-8 protein production in KGN cells, whereas there were no effects on the IL-8 protein production. in GLCs. On the other hand, IL-8 protein production was not influenced by leptin in KGN cells and GCLs. The expression of IL-8 in GLCs and KGN cells was increased by addition of IL-1beta and TNFalpha. Interleukin-8 increased the proliferation of ovarian stromal cells. The expression of pIkappaB in KGN cells was induced by IL-1beta, and the effects were reduced by APDC. Interleukin 8 induced by IL-1beta via activation of NF-kappaB in granulosa cells may have a role in the periovulatory period of follicular maturation.

我们评估了瘦素和白细胞介素（IL-8）在人卵巢中的表达、调控和作用。在IVF周期中收集卵泡液和颗粒叶黄素细胞（GLCs）。还使用了人颗粒细胞肿瘤细胞系KGN细胞。ELISA法测定大鼠卵泡液中瘦素、IL-8水平及IL-8蛋白含量。卵泡液中瘦素水平与白细胞介素-8水平呈正相关。逆转录聚合酶链反应显示GLCs和KGN细胞中存在瘦素受体mRNA和IL-8 mRNA。相反，在这些细胞中未检测到IL-8受体mRNA。E2和FSH的加入显著增加了KGN细胞IL-8蛋白的产生，而对IL-8蛋白的产生没有影响。在GLCs。另一方面，在KGN细胞和gcl中，IL-8蛋白的产生不受瘦素的影响。在GLCs和KGN细胞中添加il -1 β和TNFalpha可增加IL-8的表达。白细胞介素-8增加卵巢间质细胞的增殖。il -1 β可诱导KGN细胞中pIkappaB的表达，而APDC可减弱其作用。il -1 β通过活化颗粒细胞中的NF-kappaB诱导的白细胞介素8可能在卵泡成熟的排卵期起作用。

项目成果

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