The molecular mechanism and morphological change of oncogenesis of choriocarcinoma

绒毛膜癌发生的分子机制及形态学变化

• 批准号: 13671726
• 负责人: FUJITA Takuji
• 金额: $ 2.37万
• 依托单位: Kyushu University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2001
• 资助国家: 日本
• 起止时间: 2001 至 2002
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-13671726/
• 关键词: Choriocarcinoma; Fas Ligand; TNF-α; Rcasl; Edg; Fas; Apoptosis; VEGF; Rcas1

To clarify the mechanism of canceration from trophoblastic disease to choriocarcinoma, we examined expressions of TNF-α, Rcas1, Fas Ligand in normal villi, hydatidiform mole, invasive mole, and choriocarcinoma. The results are as follows: (1) The expression of Fas Ligand, which appears in normal villi, is disappeared in circumferential proliferation of hydatidiform mole. (2) No expression of Fas Ligand in invasive mole and choricarcinoma. (3) No expression of TNF-α is observed in normal villi, hydatidiform mole, invasive mole, and choriocarcinoma. (4) No expression of Rcas1 is observed in normal villi, hydatidiform mole. (5) The expression of Rcas1 is observed in invasive mole and choriocarcinoma. These results suggested that apoptosis induced factors may involve in canceration from trophoblastic disease to choriocarcinoma.Apoptosis induced factors, TNF-α, Rcas1, and Fas Ligand are secreted by shedding on cell membrane. The expressions of TNF-α, Rcas1, and Fas Ligand are controlled by … More G Protein Coupled Receptor (GRCR) on cell membrane. Edg2 receptor (endothelial differentiation gene -2 rec eptor), one of GRCR, which is observed high expression in chorionic villi, play a central role of angiogenesis. In view of the above, we try to investigate expressions of Edg2, Edg4, and Edg7 in normal villi, hydatidiform mole, invasive mole, and choriocarcinoma. Firstly to make clear about the biological significance of Edgs, we analyzed the alterations of Edg2, Edg4, and Edg7 from non-cancerous state to cancerous state. The results are as follows: (1) The expression of Edg2 is decreased and the expressions of Edg4 and Edg7 are increased from non-cancerous state to cancerous state. (2) We analyzed the correlation coefficiency in the expression between Edg2, Edg4, or Edg7 and angiogenetic factors, IL-8 or VEGF. A significant corr elation is found in the expression between Edg4 and VEGF (γ=0.613, P<0.0001). The correlation coefficient in the expression between Edg7 and VEGF (γ=0.434, P<0.001). These results suggested that signals mediated by Edgs may be involved in production of angiogenetic facto rs and cancer behavior. Less

为探讨滋养细胞疾病向绒毛膜癌癌变的机制，我们检测了TNF-α、Rcas 1、Fas Ligand在正常绒毛、葡萄胎、侵蚀性葡萄胎和绒毛膜癌中的表达。结果如下：（1）正常绒毛组织中Fas配体的表达在葡萄胎周周向增生组织中消失。(2)Fas配体在侵蚀性葡萄胎和脉络膜癌中无表达。(3)TNF-α在正常绒毛、葡萄胎、侵蚀性葡萄胎和绒毛膜癌中均无表达。(4)Rcas 1在正常绒毛、葡萄胎中无表达。(5)Rcas 1在侵蚀性葡萄胎和绒毛膜癌中均有表达。这些结果提示，从滋养细胞疾病到绒毛膜癌的癌变过程中可能存在凋亡诱导因子，TNF-α、Rcas 1和Fas Ligand通过脱落在细胞膜上而分泌。TNF-α、Rcas 1和Fas配体的表达受 ...更多信息 G蛋白偶联受体（GRCR）位于细胞膜上。Edg 2受体（endothelial differentiation gene-2 receptor）是GRCR中的一种，在绒毛组织中高表达，在血管生成中起重要作用。鉴于此，我们试图研究Edg 2、Edg 4和Edg 7在正常绒毛、葡萄胎、侵蚀性葡萄胎和绒毛膜癌中的表达。首先，为了明确Edgs的生物学意义，我们分析了Edg 2，Edg 4和Edg 7在非癌状态到癌状态的变化。结果如下：（1）从非癌状态到癌状态，Edg 2的表达减少，Edg 4和Edg 7的表达增加。(2)我们分析了Edg 2、Edg 4或Edg 7与血管生成因子IL-8或VEGF之间表达的相关系数。Edg 4与VEGF的表达呈显著正相关（γ=0.613，P<0.0001）。Edg 7与VEGF表达的相关系数r =0.434，P<0.001。这些结果提示Edgs介导的信号可能参与了血管生成因子的产生和肿瘤的行为。少

项目成果

Shingo Miyamoto, et al.: "Loss of Motility-Related Protein 1 (MRP1/CD9) and Integrin α 3 Expression in Endometrial Cancers"Cancer. 92. 542-548 (2001)

Shingo Miyamoto 等人：“子宫内膜癌中运动相关蛋白 1 (MRP1/CD9) 和整合素 α 3 表达的丢失”癌症。 92. 542-548 (2001)

Kenzo Sonoda, Shingo Miyamoto et al.: "The Clinical Significance and Function of Tumor-Associated Antigen RCAS1"Proceedings : The 12^<th> Fukuoka International Symposium on Perinatal Medicine. 12. 66-69 (2001)

Kenzo Sonoda、Shingo Miyamoto 等：“肿瘤相关抗原 RCAS1 的临床意义和功能”论文集：第 12 届福冈国际围产期医学研讨会。

Ben-Zion Katz, Shingo Miyamoto, et al.: "Direct transmembrane clustering and cytoplasmic dimerization of focal adhesion kinase initiates its tyrosine phosphorylation"Biochimica et Biophysica Acta. 1592. 141-152 (2002)

Ben-Zion Katz、Shingo Miyamoto 等人：“粘着斑激酶的直接跨膜聚类和细胞质二聚化启动其酪氨酸磷酸化”Biochimica et Biophysicala Acta。

Akiko Maruyama, Shingo Miyamoto et al.: "Clinicopathologic and Familial Characteristics of Endometrial Carcinoma with Multiple Primary Carcinomas relation to the loss of Protein Expression of MSH2 and MLH1"Cancer. 91. 2056-2064 (2001)

Akiko Maruyama、Shingo Miyamoto 等人：“子宫内膜癌伴多原发癌的临床病理学和家族特征与 MSH2 和 MLH1 蛋白表达缺失的关系”癌症。

Shingo Miyamoto, Akiko Maruyama, Kaoru Okugawa, Kohei Akazawa, Hideo Baba, Yoshihiko Maehara, Eisuke Mekada.: "Loss of Motility-Related Protein 1 (MRP1/CD9) and Integrin α3 Expression in Endometrial Cancers"Cancer. 92. 542-548 (2001)

Shingo Miyamoto、Akiko Maruyama、Kaoru Okukawa、Kohei Akazawa、Hideo Baba、Yoshihiko Maehara、Eisuke Mekada.：“子宫内膜癌中动力相关蛋白 1 (MRP1/CD9) 和整合素 α3 表达的丧失”癌症。 (2001)