Study of the cyclic ADP-ribose signal system in the olfactory pathway

嗅觉通路中环状ADP-核糖信号系统的研究

• 批准号: 13671773
• 负责人: MIWA Takaki
• 金额: $ 2.62万
• 依托单位: Kanazawa University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2001
• 资助国家: 日本
• 起止时间: 2001 至 2002
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-13671773/
• 关键词: cyclic ADP-ribose; Olfactory pathway; signal transduction; olfactory receptor cell; mouse; セカンドメッセンジヤー

It has been believed that olfactory transduction is mediated by two intracellular pathways, cAMP and IP3-pathways. These pathways are mediated by G-coupled protein. Recent studies have indicated that cyclic ADP-ribose (cADPR) related with signal systems in both insulin secretion from pancreas and signal transduction in Purkinje's cells of cerebellum. It is the purpose of this research to make inquiries into the relation of cADPR with signal transduction in olfactory receptor cells. To resolve this, we investigated them using olfactory epithelium of mouse by molecular biological technique. Cyclic AMP and cADPR concentrations of the extract from olfactory cilia fragments were measured by modified calcium shock method, to which evaluate in an instant of olfactory stimulation, but no remarkable changes were gained. The ligand binding of olfactory molecule to its receptor is vanished in an instant. More ingenious experiment system are needed to measure the instantaneous response in olfactory system.

嗅觉信号转导被认为是由两条细胞内途径，cAMP和IP 3-途径介导的。这些途径由G偶联蛋白介导。近年来的研究表明，环腺苷二磷酸核糖（cADPR）与胰腺胰岛素分泌和小脑浦肯野细胞信号转导的信号系统有关。本研究的目的是探讨cADPR与嗅觉受体细胞信号转导的关系。为了解决这一问题，我们采用分子生物学技术对小鼠嗅上皮进行了研究。用改良的钙休克法测定嗅纤毛碎片提取液中cAMP和cADPR的浓度，以评价嗅刺激的瞬间效应，但未见明显变化。嗅觉分子与受体的配体结合瞬间消失。嗅觉系统的瞬时响应的测量需要更加精巧的实验系统。

项目成果

三輪高喜: "嗅覚障害の診断 現状と展望"日本味と匂学会誌. 10. 59-66 (2003)

Takaki Miwa：“嗅觉障碍的诊断：现状和前景”日本味觉学会杂志 10. 59-66 (2003)。

三輪高喜: "アロマサイエンスシリーズ21 ニオイの受容"フレグランスジャーナル. 272 (2002)

Takaki Miwa：“芳香科学系列21气味接收”香水杂志272。

Miwa T et al.: "Role of nerve growth factor in the olfactory epithelium"Microscopy Research and Technique. 58. 197-203 (2002)

Miwa T 等人：“神经生长因子在嗅觉上皮中的作用”显微镜研究和技术。

TAKAKI MIWA: "Role of nerve growth factor in the olfact ory epithelium"Microscopy Research and Technique. 58. 197-203 (2002)

TAKAKI MIWA：“神经生长因子在嗅觉上皮细胞中的作用”显微镜研究和技术。