Study of the cyclic ADP-ribose signal system in the olfactory pathway

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It has been believed that olfactory transduction is mediated by two intracellular pathways, cAMP and IP3-pathways. These pathways are mediated by G-coupled protein. Recent studies have indicated that cyclic ADP-ribose (cADPR) related with signal systems in both insulin secretion from pancreas and signal transduction in Purkinje's cells of cerebellum. It is the purpose of this research to make inquiries into the relation of cADPR with signal transduction in olfactory receptor cells. To resolve this, we investigated them using olfactory epithelium of mouse by molecular biological technique. Cyclic AMP and cADPR concentrations of the extract from olfactory cilia fragments were measured by modified calcium shock method, to which evaluate in an instant of olfactory stimulation, but no remarkable changes were gained. The ligand binding of olfactory molecule to its receptor is vanished in an instant. More ingenious experiment system are needed to measure the instantaneous response in olfactory system.