Basic research for olfactory nerve transplantation in mice

目的：探讨嗅觉上皮移植治疗嗅觉障碍的新方法。研究设计：取供体小鼠嗅黏膜，植入受体小鼠鼻腔。供体为不同的条件组织(前、成体或成熟的、完整或切片的粘膜、基底细胞的提取液)。受体小鼠存活7~28天。用抗蛋白基因产物9.5和嗅觉标记蛋白抗体对移植组织进行免疫组织化学分析。结果：受者鼻腔内无存活的嗅神经元。目前认为异物的炎症或排斥反应抑制了移植组织的存活。

Objective: To develop a new treatment for olfactory disturbance transplantation of olfactory epithelium attempted to mice. Study design: Olfactory mucosa pick off donor mouse was inserted into nasal cavity of recipient mouse. Varied conditioned tissues were used for donor (ex, adult or in matured, whole or sliced mucosa, extract of basal cells). Recipient mouse was allowed to survive for 7 to 28days. An immunohistochemical analysis of transplanted tissue was done using both anti protein gene product 9.5 and olfactory marker protein antibody. Results: There were no survived olfactory neuron in recipient's nasal cavity. It is suspected that inflammation or rejection for foreign body inhibited the survive of transplanted tissue.