Basic research for olfactory nerve transplantation in mice

目的：探索嗅上皮移植治疗嗅觉障碍的新方法。研究设计：将供体小鼠的嗅粘膜挑取物插入受体小鼠的鼻腔中。各种条件组织用于供体（例如，成人或成熟的，完整或切片的粘膜，基底细胞提取物）。使小鼠存活7 - 28天。免疫组化分析移植组织使用抗蛋白基因产物9.5和嗅觉标记蛋白抗体。结果：受体鼻腔内无嗅神经元存活。可能是炎症反应或异物排斥反应抑制了移植组织的存活。

Objective: To develop a new treatment for olfactory disturbance transplantation of olfactory epithelium attempted to mice. Study design: Olfactory mucosa pick off donor mouse was inserted into nasal cavity of recipient mouse. Varied conditioned tissues were used for donor (ex, adult or in matured, whole or sliced mucosa, extract of basal cells). Recipient mouse was allowed to survive for 7 to 28days. An immunohistochemical analysis of transplanted tissue was done using both anti protein gene product 9.5 and olfactory marker protein antibody. Results: There were no survived olfactory neuron in recipient's nasal cavity. It is suspected that inflammation or rejection for foreign body inhibited the survive of transplanted tissue.