Basic research for olfactory nerve transplantation in mice

小鼠嗅神经移植的基础研究

• 批准号: 10671586
• 负责人: MIWA Takaki
• 金额: $ 2.11万
• 依托单位: Kanazawa University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1998
• 资助国家: 日本
• 起止时间: 1998 至 2000
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-10671586/
• 关键词: transplantation; olfactory neuron; immnohistochemistry; 嗅細胞移植; P2 mice; Rosa mice; LacZ; olfactory marker protein; Olfactory marker protein; Balb-Cマウス

Objective: To develop a new treatment for olfactory disturbance transplantation of olfactory epithelium attempted to mice. Study design: Olfactory mucosa pick off donor mouse was inserted into nasal cavity of recipient mouse. Varied conditioned tissues were used for donor (ex, adult or in matured, whole or sliced mucosa, extract of basal cells). Recipient mouse was allowed to survive for 7 to 28days. An immunohistochemical analysis of transplanted tissue was done using both anti protein gene product 9.5 and olfactory marker protein antibody. Results: There were no survived olfactory neuron in recipient's nasal cavity. It is suspected that inflammation or rejection for foreign body inhibited the survive of transplanted tissue.

目的：探讨嗅觉上皮移植治疗嗅觉障碍的新方法。研究设计：取供体小鼠嗅黏膜，植入受体小鼠鼻腔。供体为不同的条件组织(前、成体或成熟的、完整或切片的粘膜、基底细胞的提取液)。受体小鼠存活7~28天。用抗蛋白基因产物9.5和嗅觉标记蛋白抗体对移植组织进行免疫组织化学分析。结果：受者鼻腔内无存活的嗅神经元。目前认为异物的炎症或排斥反应抑制了移植组织的存活。

项目成果

Nishimura T, Miwa T, Furukawa M et al: "Glucocorticoid enhances Na/K ATPase mRNA expression in rat olfactory mucosa during regeneration"Chemical Senses. 27. 13-22 (2002)

Nishimura T、Miwa T、Furukawa M 等人：“糖皮质激素在再生过程中增强大鼠嗅粘膜中 Na/K ATP 酶 mRNA 的表达”Chemical Senses。

三輪高喜他: "Impact of olfactory impairment quality of life and disability"Arch. Otolaryugol. Head Neck Surg.. 127. 497-503 (2001)

Takaki Miwa 等人：“嗅觉障碍对生活质量和残疾的影响”Arch. Otolaryugol. 127. 497-503 (2001)

西村俊郎他: "Glucocorticoid enhances Na^+/K^+ATpase mRNA expression in rat olfactory mucosa during regeneration"Chemical Senses. 27. 13-22 (2002)

Toshiro Nishimura 等人：“糖皮质激素在再生过程中增强大鼠嗅粘膜中的 Na^+/K^+ATpase mRNA 表达”Chemical Senses。27. 13-22 (2002)

三輪高喜他: "TrkA expression in mouse olfactory tract following anotomy of olfactory nerv"Acta Otolaryngol. Suppl. 539. 79-82 (1998)

Takaki Miwa 等：“嗅神经解剖后小鼠嗅道中的 TrkA 表达”Acta Otolaryngol. 539. 79-82 (1998)

三輪高喜他: "The role of nerve growth factor in the olfactory system"Microscopy Research and Teckinigue. (印刷中). (2002)

Takaki Miwa 等人：“神经生长因子在嗅觉系统中的作用”显微镜研究和 Teckinigue（印刷中）。