Basic research for olfactory nerve transplantation in mice

目的：探讨小鼠嗅上皮移植治疗嗅障碍的新方法。研究设计：将供鼠嗅粘膜摘取物插入受体鼠鼻腔。不同的条件组织被用于供体（例如，成人或成熟，整个或切片粘膜，基底细胞提取物）。受体小鼠存活7 ~ 28天。采用抗蛋白基因产物9.5和嗅觉标记蛋白抗体对移植组织进行免疫组化分析。结果：受体鼻腔内未见嗅神经元存活。怀疑是炎症或异物排斥抑制了移植组织的存活。

Objective: To develop a new treatment for olfactory disturbance transplantation of olfactory epithelium attempted to mice. Study design: Olfactory mucosa pick off donor mouse was inserted into nasal cavity of recipient mouse. Varied conditioned tissues were used for donor (ex, adult or in matured, whole or sliced mucosa, extract of basal cells). Recipient mouse was allowed to survive for 7 to 28days. An immunohistochemical analysis of transplanted tissue was done using both anti protein gene product 9.5 and olfactory marker protein antibody. Results: There were no survived olfactory neuron in recipient's nasal cavity. It is suspected that inflammation or rejection for foreign body inhibited the survive of transplanted tissue.