Study of the expressions of differenciation factors to olfactory neuron in the olfactory epithelium of human and the other animal.

本研究的目的是证实人类嗅粘膜中一些与分化为成熟嗅神经元相关的因子的表达。在患者同意下用特制镊子去除的嗅粘膜分为三种类型：存在嗅神经细胞、仅存在呼吸柱细胞和由于机械伪影而缺失任何上皮细胞。通过免疫组织化学研究，在嗅觉上皮细胞中观察到一些神经标记物。然而，在该实验系统中的人嗅上皮和培养组织中没有发现任何与神经分化相关的标志物。看来嗅上皮的去除、固定和组织培养过程还需要更多的匠心。

The purpose of this research was to prove the expression of some factors related with differentiation to the mature olfactory neuron in human olfactory mucosa. The olfactory mucosa removed by a special forceps under the patient's agreement were divided three types, existing olfactory neural cells, existing only respiratory column cells and missing any epithelial cells by the mechanical artifacts. Some neural markers were observed-sparsely in the olfactory epithelium by immunohistochemical study. However, any markers related with neural differentiation were not found in the human olfactory epithelium and cultured tissue in this experiment system. It is seemed that more ingenuity is needed at the course of removal, fixation and tissue culture of olfactory epithelium.