Study of the expressions of differenciation factors to olfactory neuron in the olfactory epithelium of human and the other animal.

本研究的目的是证实一些与成熟嗅神经元分化相关的因子在人嗅黏膜中的表达。在患者的同意下，用特殊的钳子取出的嗅黏膜分为三种类型，即存在嗅神经细胞、仅存在呼吸柱细胞和因机械假体而丢失任何上皮细胞。免疫组织化学显示嗅觉上皮内有少量神经标记物。然而，在本实验系统中，人嗅觉上皮和培养组织中没有发现任何与神经分化有关的标志物。在嗅上皮的去除、固定和组织培养过程中，似乎还需要更多的巧妙。

The purpose of this research was to prove the expression of some factors related with differentiation to the mature olfactory neuron in human olfactory mucosa. The olfactory mucosa removed by a special forceps under the patient's agreement were divided three types, existing olfactory neural cells, existing only respiratory column cells and missing any epithelial cells by the mechanical artifacts. Some neural markers were observed-sparsely in the olfactory epithelium by immunohistochemical study. However, any markers related with neural differentiation were not found in the human olfactory epithelium and cultured tissue in this experiment system. It is seemed that more ingenuity is needed at the course of removal, fixation and tissue culture of olfactory epithelium.