Study of the expressions of differenciation factors to olfactory neuron in the olfactory epithelium of human and the other animal.

本研究的目的是证实人嗅粘膜中与嗅神经元分化相关的一些因子的表达。在患者同意的情况下用特殊镊子取出嗅粘膜，将其分为三种类型，即存在嗅神经细胞、仅存在呼吸柱细胞和因机械伪影而缺失任何上皮细胞。免疫组化染色显示嗅上皮内有少量神经标记物。但在本实验系统培养的人嗅上皮及组织中未发现与神经分化相关的标记物。在嗅上皮的切除、固定和组织培养过程中，似乎需要更多的技巧。

The purpose of this research was to prove the expression of some factors related with differentiation to the mature olfactory neuron in human olfactory mucosa. The olfactory mucosa removed by a special forceps under the patient's agreement were divided three types, existing olfactory neural cells, existing only respiratory column cells and missing any epithelial cells by the mechanical artifacts. Some neural markers were observed-sparsely in the olfactory epithelium by immunohistochemical study. However, any markers related with neural differentiation were not found in the human olfactory epithelium and cultured tissue in this experiment system. It is seemed that more ingenuity is needed at the course of removal, fixation and tissue culture of olfactory epithelium.