Analysis of MIF function in wound healing using transgenic mouse

转基因小鼠MIF在伤口愈合中的功能分析

• 批准号: 13671874
• 负责人: SASAKI Satoru
• 金额: $ 2.62万
• 依托单位: HOKKAIDO UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2001
• 资助国家: 日本
• 起止时间: 2001 至 2002
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-13671874/
• 关键词: macrophage migration inhibitory factor; transgenic mouse; wound healing; keloid; siRNA; extracellular matrix; cellular proliferation; アポトーシス; 細胞周期

It has been shown that macrophage migration inhibitory factor (MIF) plays a key role in wound repair based on the results on in vitro and in vitro studies. Keloids form when the normal wound-healing process is dysregulated and the evolving scar remains in the proliferative phase of healing.In this study, we reported for the first time that fibroblasts of the keloid express high MIF mRNA and produce MIF protein. Immunohistochemocal analysis demonstrated that MIF was mostly localized in the cytoplasm of fibroblast. To assess the role of MIF overexpression in keloid, short interferring RNA (siRNA) for MIF was transfected to keloid-derived fibroblasts. The results demonstrated that the cell growth rate and expression of type I collagen mRNA were markedly suppressed. Taken together, these results indicated that it is likely that MIF may function as a novel growth factor that stimulates incessant growth and extracellular matrix production of fibroblast of keloid. MIF plays an important role in keloidgenesis.

巨噬细胞移动抑制因子（macrophage migration inhibitor factor，MIF）在创伤修复中起着重要作用。瘢痕疙瘩形成时，正常的伤口愈合过程中失调，不断发展的瘢痕仍然处于增殖阶段的healing.In这项研究中，我们首次报道，瘢痕疙瘩的成纤维细胞表达高MIF mRNA和产生MIF蛋白。免疫组化显示MIF主要定位于成纤维细胞的胞浆内。为了评估MIF过表达在瘢痕疙瘩中的作用，将MIF的短干扰RNA（siRNA）转染瘢痕疙瘩来源的成纤维细胞。结果表明，细胞生长速率和I型胶原mRNA的表达明显受到抑制。这些结果表明，MIF可能作为一种新的生长因子，刺激瘢痕疙瘩成纤维细胞的不断生长和细胞外基质的产生。MIF在瘢痕疙瘩的形成中起重要作用。

项目成果

小山明彦: "ケロイドにおけるマクロファージ遊走阻止因子の過剰発現と病態に果たす役割"北海道医学雑誌. (印刷中). (2003)

小山明彦：“疤痕疙瘩中巨噬细胞迁移抑制因子的过度表达及其在病理学中的作用”北海道医学杂志（2003 年出版）。