Nerve regeneration tracing using adenovirus vector after end-to-side nerve coaptation

端侧接合后神经纤维再生。这些再生纤维通常被认为是从受体神经的节间空间发芽。用含有LacZ基因的重组腺病毒载体，对于周围神经纤维的追踪，我们表明再生的神经纤维可以沿着其长度追踪。在以往的实验中，腓神经与胫神经端侧吻合，再生纤维仅从腓神经原神经元近端长出，而受体神经无明显侧支结长出;在本实验中，将切断的肌皮神经与正中神经或尺神经端侧吻合。在正中神经受体组中，大多数再生纤维远离近端向下。在尺神经受体组中，如果从尺神经的C7臂丛神经根没有明显的通路，则会发生明显的结间出芽，并且大多数纤维从端侧接合部位向下出芽，这表明结间出芽来自受体神经纤维。如果C7神经根与尺神经之间有交通分支，则再生纤维混合型直接进入吻合的肌皮神经段或从受神经处形成T形出芽纤维。本研究结果提示，在端侧神经修复中，神经的向神经性可以使神经纤维再生，如从受损神经的原始神经元中长出纤维，而不是从受体神经的节间长出纤维。

Nerve fibers regenerate after end-to-side coaptation. These regenerating fibers have been believed often to sprout from the inter-nodal space of the recipient nerve. With a recombinant adenovirus vector containing the LacZ gene, for the tracing of the peripheral nerve fibers we showed that the regenerating nerve fibers can be traced along its length. In the previous study in which the peroneal nerve was coapted end-to-side to the tibial nerve, the regenerating fibers sprouted from just proximal to the original neuron of the peroneal nerve, without discernible collateral nodal sprouting from the recipient nerve.In the present study, the severed musculocutaneous nerve was coapted end-to-side connected to the median or ulnar nerve in end to side manner. In the median nerve recipient group, most of the regenerating fibers were coming down far from proximally. In the ulnar nerve recipient group, demonstrated internodal sprouting occurred if there was no demonstrable pathway from the C7 brachial plexus root of the ulnar nerve, and most of the fibers sprouted from going down through the end-to-side coaptation site, suggesting that the internodal sprouting came from the recipient nerve fiber. If there was a communicating branch between the C7 root and the ulnar nerve, mixture patterns of the regenerating fibers directly going either into the coapted musculocutaneous nerve segment and or formed a T-shaped sprouting fiber from the recipient nerve. Fibers were demonstrated if there was a communicating branch between C7 and ulnar nerve.This study suggests novel facts that in end-to-side nerve repair, neurotropism to attain more reasonable nerve fibers may regeneration such as sprouting fibers from the original neurons of the injured nerve, rather than by the internodal sprouting from the recipient nerve may work in end-to-side nerve repair.