Investigation of nerve regeneration and its plasticity using a new antibodies against phosphorylated GAP-43 (p-GAP-43)

观察端侧神经吻合后周围神经的组织学变化，以确定术中神经束膜开放和故意损伤供体神经的影响。将肌皮（受体）神经与尺（供体）神经接合后7天，使用抗neuroadherent-H（RT 97）和磷酸化GAP-43（p-GAP-43）的抗体对神经进行免疫化学分析。后者被发现专门检测生成神经纤维。统计学评价显示，神经束膜开放和故意供体神经损伤在端侧神经接合中的疗效增加，这表明部分神经纤维疝出伴部分轴突断伤或神经切断对于端侧神经接合中的有效轴突再生很重要。

Histological changes were observed in peripheral nerves following end-to-side nerve coaptation to determine the effects of perineurial opening and deliberate donor nerve injury during surgery. Seven days after coaptation of the musculocutaneous (recipient) nerve to the ulnar (donor) nerve, the nerves were immunohistochemically analysed using antibodies against neurofilament-H (RT97) and phosphorylated GAP-43 (p-GAP-43). The latter was found to detect specifically generating nerve fibers. Statistical evaluation revealed increased efficacy of perineurial opening and deliberate donor nerve injury in end-to-side nerve coaptation, suggesting that partial nerve fiber herniation with partial axonotmesis or neurotomesis was important for effective axonal regeneration in end-to-side nerve coaptation.