Investigation of nerve regeneration and its plasticity using a new antibodies against phosphorylated GAP-43 (p-GAP-43)

观察端侧神经吻合后周围神经的组织学变化，以确定手术中神经周围神经开放和故意供体神经损伤的影响。肌皮神经与尺神经吻合7天后，用抗神经丝蛋白-H(RT97)和磷酸化生长相关蛋白-43(p-GAP-43)的抗体进行免疫组织化学分析。后者被发现是专门检测产生神经纤维的。统计分析显示，神经外膜切开和故意损伤供体神经在端侧神经吻合中的有效性增加，提示部分神经纤维突出合并部分轴突或神经切断对于端侧神经吻合中有效的轴突再生是重要的。

Histological changes were observed in peripheral nerves following end-to-side nerve coaptation to determine the effects of perineurial opening and deliberate donor nerve injury during surgery. Seven days after coaptation of the musculocutaneous (recipient) nerve to the ulnar (donor) nerve, the nerves were immunohistochemically analysed using antibodies against neurofilament-H (RT97) and phosphorylated GAP-43 (p-GAP-43). The latter was found to detect specifically generating nerve fibers. Statistical evaluation revealed increased efficacy of perineurial opening and deliberate donor nerve injury in end-to-side nerve coaptation, suggesting that partial nerve fiber herniation with partial axonotmesis or neurotomesis was important for effective axonal regeneration in end-to-side nerve coaptation.