Development of new pulp capping/ amputation agent combined with mineral induction activity

结合矿物质诱导活性开发新型盖髓/截肢剂

• 批准号: 10470408
• 负责人: MATSUDA Koichi
• 金额: $ 9.73万
• 依托单位: Health Sciences University of Hokkaido
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 1998
• 资助国家: 日本
• 起止时间: 1998 至 2001
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-10470408/
• 关键词: BMP; Mineral Induction; Pulp Capping; Pulp Amputation; 象牙芽細胞; 断髄剤; rhBMP-2; コラーゲン; 多孔質ハイドロキシアパタイト; phosphophoryn; BMP; 石灰化; 象牙質誘導

The final goal of this project is to develop new bio - active pulp capping agent using rhBMP-2. First, we examined the effects of rhBMP-2 on differentiation of human pulp cells by measurement of, mRNA expression using quantitative PCR method. We got pulp tissue from extracted fresh wisdom tooth, and obtained pulp cells by out-growth method. We cultured the pulp cells with rhBMP-2 of 0, 10, ; 100 and 1000 ng/ml without serum, and measured various mRNA expressions using LightCycler (Roche, Germany). As a result, rhBMP-2 enhanced dentin sialo phosphoprotein mRNA expression that is thought to be marker of odontoblast differentiation, in a concentration pendent manner. Therefore, we found that rhBMP-2 enhanced the differentiation of pulp cells into odontoblasts, and it is suggested that effective pulp-conservative agent would be produced by using rhBMP-2. Next, We implanted rhBMP-2/ type I collagen/ hydroxyapatite/ phosphophoryn composite into subcutaneous connective tissue or onto skull of rat. Then we sacrificed the rats, and analyzed the implants biochemically and histologically. As a result, we found that rhBMP-2/ type I collagen/ hydroxyapatite/ phosphophoryn composite induced bone formation strongly compared with control group. It was Indicated that rhBMP-2/ type I collagen/ hydroxyapatite/ phosphophoryn composite can be effective pulp capping agent.

本课题的最终目标是研制新型的生物活性盖髓剂.首先，我们用定量PCR方法检测rhBMP-2对人牙髓细胞分化的影响。取离体新鲜智齿的牙髓组织，采用体外生长法获得牙髓细胞。我们在无血清条件下用0、10、100和1000 ng/ml的rhBMP-2培养牙髓细胞，并使用LightCycler（Roche，德国）测量各种mRNA表达。结果表明，rhBMP-2以浓度依赖性方式增强牙本质涎磷蛋白mRNA的表达，这被认为是成牙本质细胞分化的标志。因此，我们发现rhBMP-2促进牙髓细胞向成牙本质细胞的分化，并建议使用rhBMP-2将产生有效的牙髓保存剂。然后将rhBMP-2/I型胶原/羟基磷灰石/磷酸钙复合物植入大鼠皮下结缔组织或颅骨内。然后，我们处死了老鼠，并对植入物进行了生化和组织学分析。结果表明，rhBMP-2/I型胶原/羟基磷灰石/磷酸化蛋白复合材料与对照组相比，具有较强的诱导成骨作用。rhBMP-2/I型胶原/羟基磷灰石/磷酸钙复合材料是一种有效的盖髓剂。

项目成果

豊岡 広起: "遊離型ホスホホリンによる石灰化誘導"日本歯科保存学雑誌. 42. 640-646 (1999)

Hiroki Toyooka：“游离磷蛋白诱导钙化”日本保守牙科杂志 42. 640-646 (1999)

K.Endo et al.: "Corrosion characteristics of ferric and austenitic stainless steels for dental magnetic attachment"Dental Mater. J.. 19. 34-49 (2000)

K.Endo 等人：“用于牙科磁性附件的铁和奥氏体不锈钢的腐蚀特性”Dental Mater。

T. Kawakarai, Y. Ibaraki, T. Saito, K. Matsuda: "Application of Erbium : YAG laser to comnosite resin restoration in anterior teeth"J. Esthetic Dent.. 11. 80-85 (1998)

T. Kawakarai、Y. Ibaraki、T. Saito、K. Matsuda：“铒：YAG 激光在前牙复合树脂修复中的应用”J。

S. Ito, T. saito, MA. Crenshaw, E Toyooka, K.Matsuda: "In vitro mineral induction by immobilized phosphoprotein -Effect of phosphate group on inter facial tension for mineral induction-"Higashi Nippon Dental J.. 18. 7-15 (1999)

S. Ito，T. Saito，MA。

K.Matsuda et al.: "In vivo corrosion and tarnish of dental Au-based and Ag-based alloy restorations"J. Esthetic Dent.. 11. 58-66 (1998)

K.Matsuda 等：“牙科金基和银基合金修复体的体内腐蚀和失去光泽”J。