Pathogen enzyme productivity of bacteria isolated from a therapy resistant periapical periodontitis cases

难治性根尖周炎是由粪肠球菌、白色念珠菌、铜绿假单胞菌、口腔链球菌等对根管治疗产生抗药性的细菌持续感染引起的，难治性根尖周炎多为亚急性根尖周炎，但症状多样，对抗药性细菌进行病原酶产率检测，目的明确，本研究中，这些菌株对难治性根尖周炎的临床表现有一定的影响，（ATCC 35039）和口腔链球菌（S.oralis）试验菌株均表现出产病原酶的特性，粪肠球菌（E.faecalis）表现出产硫酸软骨素酶的特性，铜绿假单胞菌（P.aeruginosa）表现出产卵磷脂酶和弱产胶原酶的特性，结果表明，这些细菌的产酶特性可能参与了难治性根尖周炎向临床症状持续发展的过程。

Refractory periapical periodontitis is caused by persistent infection by bacteria to resist for endodontic treatment such as Enterococcs faecalis, Candida albicans, Pseudomonas aeruginosa and Streptococcus oralis.Many of refractory periapical periodontitis cases are subacute cases, but those symptom is various.The bacteria which resisted therapy examined productivity in pathogen enzyme for the purpose of it was clear, and in this study, these doing the effect which therapy resistant bacteria gave to clinical manifestation expression of a refractory periapical periodontitis case.The type strain (ATCC35039) and the test strain of S.oralis did hot show pathogen enzyme production characteristics.E.faecalis showed chondoroitin sulfatase producing.P.aeruginosa showed lecithinase producing and weak producing such as collagenase, hyaluronidase and chondoroitin sulfatase in high frequency.These resultant showed the probability which pathogen enzyme production characteristics of these bacteria participated in refractory periapical periodontitis developing for clinical symptom persistently.