Studies on differences of alveolar bone in it's metabolism

在我们的研究中，RANKL蛋白在大鼠牙周组织中被证实具有免疫定位。观察到抗RANKL抗体对神经纤维样结构的免疫反应。为了鉴定RANKL免疫阳性的神经纤维样结构，我们进行了以下两个实验。RANKL免疫阳性神经纤维样结构的电镜观察。为了从形态学上研究RANKL免疫阳性的神经纤维样结构，我们使用了透射电子显微镜（TEM）。与RANKL抗体免疫反应后，作为预包埋方法，切片包埋在EPON中。用透射电镜观察得到的薄片。结果表明，与RANKL抗体的免疫反应产物位于细胞质突起表面。RANKL免疫阳性的结构被认为在形态上类似于神经纤维。用抗RANKL抗体和抗神经特异性蛋白pgp9.5抗体双染色处死wistar大鼠，切除下颌骨。脱矿后，进行免疫组织化学处理。RANKL的免疫反应位于血管周围，与PGP9.5的免疫定位接近。RANKL免疫阳性的神经纤维样结构多于PGP9.5免疫染色的神经纤维。然而，RANKL和PGP9.5的共定位并没有得到明确的证明。总之，这些结构在形态学上类似于这里没有发现的神经纤维。

In our study, immunolocalizations of RANKL protein were demonstrated in the rat periodontium. It was observed immunoreactions with the antibody against RANKL on nerve fibers-like structures. To identify nerve fibers-like structures immunopositive for RANKL, we performed the two experiments below.1. Electro-microscopic observations of nerve fiber-like structures immunopositive for RANKL.To morphologically study nerve fiber-like structures immunopositive for RANKL, we utilized transmission electron microscopy (TEM). After immunoreactions with an antibody against RANKL, as a pre-embedding method sections were embedded in EPON. The thin sections thus obtained were subjected to TEM observation. It demonstrated that the products of the immunoreactions with the antibody against RANKL were located on the surfaces of the cytoplasmic processes. Structures immunopositive for RANKL were suggested to be morphologically analogous to nerve fibers.2. Dual-staining with an antibody against RANKL and another against the nerve-specific protein PGP9.5Wistar rats were killed and their mandibles were removed. After demineralization, the samples were processed for immunohistochemistry. Immunoreaction for RANKL was shown to be located around the blood vessels, and to be close to the immunolocalization of PGP9.5. Nerve fibers-like structures immunopositive for RANKL were observed more than the nerve fibers immunostained with PGP9.5. However, the co-localization of RANKL and PGP9.5 was not clearly demonstrated.In conclusion, the structures were shown to be morphologically analogous to nerve fibers that were not identified here.