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Studies on differences of alveolar bone in it's metabolism

牙槽骨代谢差异的研究

基本信息

批准号：
13672046
负责人：
NAGAOKA Eiichi
金额：
$ 2.24万
依托单位：
Kagoshima University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2001
资助国家：
日本
起止时间：
2001 至 2002
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-13672046/
关键词：
RANKL ODF periodontium nerve osteoclast

项目摘要

In our study, immunolocalizations of RANKL protein were demonstrated in the rat periodontium. It was observed immunoreactions with the antibody against RANKL on nerve fibers-like structures. To identify nerve fibers-like structures immunopositive for RANKL, we performed the two experiments below.1. Electro-microscopic observations of nerve fiber-like structures immunopositive for RANKL.To morphologically study nerve fiber-like structures immunopositive for RANKL, we utilized transmission electron microscopy (TEM). After immunoreactions with an antibody against RANKL, as a pre-embedding method sections were embedded in EPON. The thin sections thus obtained were subjected to TEM observation. It demonstrated that the products of the immunoreactions with the antibody against RANKL were located on the surfaces of the cytoplasmic processes. Structures immunopositive for RANKL were suggested to be morphologically analogous to nerve fibers.2. Dual-staining with an antibody against RANKL and another against the nerve-specific protein PGP9.5Wistar rats were killed and their mandibles were removed. After demineralization, the samples were processed for immunohistochemistry. Immunoreaction for RANKL was shown to be located around the blood vessels, and to be close to the immunolocalization of PGP9.5. Nerve fibers-like structures immunopositive for RANKL were observed more than the nerve fibers immunostained with PGP9.5. However, the co-localization of RANKL and PGP9.5 was not clearly demonstrated.In conclusion, the structures were shown to be morphologically analogous to nerve fibers that were not identified here.

在我们的研究中，RANKL蛋白在大鼠牙周组织中的免疫定位得到证实。在神经纤维样结构上观察到抗RANKL抗体的免疫反应。为了鉴定RANKL免疫阳性的神经纤维样结构，我们进行了以下两个实验。RANKL免疫阳性的神经纤维样结构的电镜观察为了从形态学上研究RANKL免疫阳性的神经纤维样结构，我们使用了透射电子显微镜（TEM）。在与抗RANKL抗体免疫反应后，作为预包埋方法，将切片包埋在EPON中。对由此获得的薄切片进行TEM观察。这表明与抗RANKL抗体的免疫反应产物位于胞质突起的表面。RANKL免疫阳性结构在形态上与神经纤维相似.用抗RANKL的抗体和另一种抗神经特异性蛋白PGP9.5的抗体进行双重染色。脱矿后，对样本进行免疫组织化学处理。RANKL的免疫反应显示位于血管周围，并且接近PGP9.5的免疫定位。RANKL免疫阳性的神经纤维样结构多于PGP9.5免疫染色的神经纤维。然而，RANKL和PGP9.5的共定位没有被清楚地证明。总之，这些结构显示出在形态上类似于此处未鉴定的神经纤维。