权益分类	功能权益	普通用户	{{item.name}}会员
{{category.name}}	{{benefitItem.name}}

Application of 5-aminorevulinic acid for diagnosis and treatment of oral cancer

5-氨基乙酰丙酸在口腔癌诊治中的应用

基本信息

批准号：
13672080
负责人：
ONIZAWA Kojiro
金额：
$ 2.24万
依托单位：
University of Tsukuba
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2001
资助国家：
日本
起止时间：
2001 至 2003
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-13672080/
关键词：
Fluorescence Oral Cancer Aminorevulinic acid Photodynamic Therapy Autofluorescence

项目摘要

This study was carried out to investigate application of 5-anunorevulinic acid (ALA) for diagnosis and treatment of oral cancer. The relationship between autofluorescence and AL.A induced fluorescence was analyzed biochemically, and effect of photodynamic therapy using AIA on nude mouse tumor was evaluated histologically. The autofluorescence arising in human oral cancer was reported to be associated with protoporphyrin, and similar poiphyrins were observed in oral cancers induced in hamsters and oral-cancer cell lines. After topical or systemic administration of ALA, red fluorescence which was similar to autofluorescence in human cancer was observed in oral mucosa and cancer induced in hamster. Red fluorescence was also observed in slap and tumor formed by human oral-cancer cell lines under administration of AIA. The fluorescent substance associated with ALA was suggested to be protoporphyrin, which was consistent with those caused autofluorescence in human cancer. The enhanced intensity of red fluorescence was also demonstrated in cancer tissues of hamster or nude mouse under administration of ALA, and ALA could be applied to fluorescence diagnosis for oral cancer to enhance intensity of autofluorescence in early-staged or small-sized cancer. Although ALA-based photodynamic therapy was evaluated in nude mouse honor under various experimental conditions, tumor necrosis was observed in only superficial area and nodular tumor did not show any regression after the therapy. Further experiments would be necessary before clinical application of ALA-based photodynamic therapy for nodular cancer tissue

本研究旨在探讨5-氨基酮戊酸（ALA）在口腔癌诊断和治疗中的应用。用生物化学方法分析AIA诱导荧光与自体荧光的关系，并用组织学方法评价AIA光动力疗法对裸鼠肿瘤的治疗效果。据报道，人类口腔癌中产生的自发荧光与原卟啉有关，在仓鼠和口腔癌细胞系中诱导的口腔癌中观察到类似的卟啉。局部或全身给予ALA后，在口腔粘膜和仓鼠诱发的癌症中观察到类似于人类癌症自发荧光的红色荧光。在人口腔癌细胞系形成的瘤样组织和肿瘤中也观察到AIA的红色荧光。与ALA相关的荧光物质被认为是原卟啉，这与人类癌症中引起自发荧光的物质一致。ALA还可增强金黄地鼠和裸鼠口腔癌组织中的自体荧光强度，可用于口腔癌的荧光诊断，增强早期或小型口腔癌的自体荧光强度。尽管在各种实验条件下在裸鼠荣誉中评估了基于ALA的光动力疗法，但仅在浅表区域观察到肿瘤坏死，并且结节性肿瘤在治疗后未显示任何消退。ALA光动力治疗结节性癌组织的临床应用还需进一步的实验研究