Cell-biological study for Biological production of Proteoglycans in the Articular-disc in the case of Internalderangement Temporomandibular Joint

颞下颌关节内部紊乱情况下关节盘中蛋白多糖生物产生的细胞生物学研究

• 批准号: 13672111
• 负责人: FUJITA Shigeyuki
• 金额: $ 2.62万
• 依托单位: Wakayama Medical University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2001
• 资助国家: 日本
• 起止时间: 2001 至 2002
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-13672111/
• 关键词: internalderangement; temporomandibular joint; keratan-sulfate; RT-PCR; MMP; chondroitin-6 sulfate; COX; 顎間節内障; 家兎; 顎間節円板; プロテオグリカン; 低酸素; 1L-1Β; ケラタン硫酸; 顎関節内障; 滑膜; VEGF; コンドロイチン硫酸

Many patients who are suffering from pain and disorder due to internalderangement on temporomandibular joint (TMJ) visited our clinic but the etiology still unclear. At first, in Anatomy institute, Tubingen university Germany, I researched, in the completely internalderangement deformed articular disc, high concentration of keratan-sulfate and chondroitin-6 sulfate were identified in the antero-inferior part of the disc and hyperplastic synovial membrane was identified in these cases. In the clinical internalderangement cases, Tenascin m-RNA was recognized in the hyperplastic synovial membrane which means remodeling and reproduction were occurred. In such cases, COX-1 and COX-2 were distributed near the vascular vessels in the hyperplastic synovial membrane.We also established the synovial cell line from internalderangement TMJ cases, with the stimulation of hypoxia and Inter-leukin-1b, the cell line autocrined MMP-1, MMP-3, MMP-9 and MMP-13 by RT-PCR but with the supernnat of the culture cells, there was the decrease of concentration of keratan sulfate in the synovial cells by ELISA.

临床上因颞下颌关节内紊乱引起的疼痛和功能紊乱的患者很多，但病因尚不清楚。首先，在德国图宾根大学解剖研究所，我研究了在完全内排列变形的关节盘中，在盘的前下部鉴定出高浓度的硫酸角质素和6硫酸软骨素，并且在这些病例中鉴定出增生的滑膜。在临床内紊乱病例中，Tenascin mRNA在增生的滑膜中被识别，这意味着发生了重塑和再生。本研究还建立了颞下颌关节内紊乱病滑膜细胞系，在低氧和考克斯-1b刺激下，RT-PCR检测细胞自分泌MMP-1、MMP-3、MMP-9和MMP-13，ELISA检测滑膜细胞中硫酸角质素浓度降低。

项目成果

Fujita S: "Variation of heads of lateral pterygoud muscle and morphology of anticalar dis of human temporomandibular joint"J Oral Rehabil. 28. 560-571 (2001)

Fujita S：“翼外肌头的变异和人类颞下颌关节前牙盘的形态”J Oral Rehabil。

YOSHIDA Hiroaki: "The expression of cyclooxygenase-2 in human temporomandibular joint samples : an immunohistochemical study"J Oral Rehabil. 29・12. 1146-1152 (2002)

YOSHIDA Hiroaki：“人类颞下颌关节样品中环氧合酶-2 的表达：免疫组织化学研究”J Oral Rehabil. 29・12（2002）。

Hiroaki Yoshida: "The distribution of cytlooxygenase-1 human temporomandiblar Joint samples an iminupohistochemical study"J Oral Rehabil. 28・6. 511-516 (2001)

Hiroaki Yoshida：“细胞加氧酶-1人类颞下颌关节样本的分布的免疫组织化学研究”J Oral Rehabil. 28・6（2001）。

Yoshida H: "The expression of cyclooxygenase-2 in human temporomandibular joint Samples : an immuhohistochemical study"J Oral Rehabil. 29. 1146-1152 (2002)

Yoshida H：“人类颞下颌关节样本中环氧合酶-2 的表达：免疫组织化学研究”J Oral Rehabil。

Yoshida H: "The distribution of cyclocxygenase-1 in human temporomandibulan joint samples : an immuhohistochemical study"J oral Rehabil. 28. 511-516 (2001)

Yoshida H：“环氧化酶-1 在人类颞下颌关节样本中的分布：一项免疫组织化学研究”J 口腔康复。