Pathological analysis of the deformed temporomandibular joint disc in epithelial-mesenchymal transition and fibrosis.

点击翻译按钮获取中文摘要

In this study, we showed that in immunohistochemistry, positive staining for lumican and hyaluronan synthase 3(HAS3) was observed in deformed temporomandibular joint(TMJ) disc, whereas fibromodulin expression was localized slightly below deformed TMJ disc surface. In cell culture experiment, the expression of lumican and HAS3 mRNA was significantly enhanced by stimulation with interleukin-1β(IL-1β), whereas there were no significant changes in the expression of fibromodulin mRNA in the presence or absence of IL-1β. In animal experiment, fibrous adhesion observed in the wild-type mice TMJ, whereas there was no fibrous adhesion in the tenascin-C knockout mice.These findings suggest that to analyze the changes in ECM structure of TMJ disc may be important for understanding fibrous induction in EMT.