Analysis for Prostaglandin E2 and Vascular Endothelial Growth Factor in the Articular Disc and Synovial Cell in case of Internalderangement of the Temporomandibular Joint

颞下颌关节内部紊乱时关节盘和滑膜细胞中前列腺素 E2 和血管内皮生长因子的分析

• 批准号: 10671872
• 负责人: FUJITA Shigeyuki
• 金额: $ 2.43万
• 依托单位: KYOTO UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1998
• 资助国家: 日本
• 起止时间: 1998 至 1999
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-10671872/
• 关键词: VEGF; COX-1; Substance-P; Synovial membrane; Prostaglandin E; Immunohisto chemistry; Temporomandibulac joint; Synovial cell; Immunchistochemistry; Temporomandibular Joint; Synovial cell; Prosta glandin E_2; シクロオキシゲナーゼ-1; シクロオキシゲナーゼ-2; 顎関節内障

In Anatomy institute, Tubingen university Germany, I researched the relation between the variation of the path way on lateral-pterygoid muscle and deformity of the articulator-disc, as the result of this research, there was no significance between them. But in the completely deformed articulator disc, high concentration of keratan-sulfate and chondroitin-6 sulfate wereidentified in the antero-inferior part of the disc and hyperplastic synovial membrane was identified in these cases.In the completely deformed articulator disc of clicical internalderangement cases, Tenascin was recognized in the hyperplastic synovial membrane and Matrix Metaro Protease-3 also found in the disc, which means remodeling and reproduction were occurred in the same time. In such cases, Substance-P and COX-1 were distributed near the vascular vessels in the hyperplastic synovial membrane, and Localization of Heat-Schock Protein-47 (HSP-47) was also specific in the antero-inner part of the disc, but the HSC-70 was identified in roard bearing part of the disc. We also established the synovial cell line from internalderangement cases, with the stimulation of Inter-leukin-1b (1ng/ml), the cell line autocrined Vascular

在德国图宾根大学解剖学研究所，我研究了翼外肌走行方式的变化与关节盘畸形的关系，结果表明，两者之间无显著性差异。而在完全变形的牙合盘中，关节盘前下部有高浓度的硫酸角质素和6硫酸软骨素，并有增生的滑膜，在典型内紊乱的完全变形的牙合盘中，增生的滑膜中有Tenascin，关节盘中也有Matrix Metaro Protease-3，也就是说重塑和繁殖是同时发生的在这些病例中，P物质和考克斯1分布在增生的滑膜中的血管附近，热休克蛋白47（HSP-47）也在椎间盘的前内部分特异性定位，但HSC-70在椎间盘的前轴承部分被鉴定。我们还建立了内分泌紊乱滑膜细胞系，在IL-1b（1 ng/ml）刺激下，

项目成果

Hiroaki Yoshida: "The distribution of cycloxygenase-1 in human Temporomandibular joint samples"Journal of Oral Rehabilitation. (In press). (1999)

Hiroaki Yoshida：“环氧化酶-1在人类颞下颌关节样本中的分布”口腔康复杂志。

Hiroaki Yoshida: "The localization of Matrix Metalloproteinase-3 and Tenascin in synovial membrane of the temporomandibula joint"Oral Disease. 5. 50-54 (1999)

Hiroaki Yoshida：“基质金属蛋白酶-3 和腱蛋白在颞下颌关节滑液膜中的定位”口腔疾病。

Hiroaki Yoshida: "Angiogenesis in the human temporomandibular Joint studied by immunohistochemistry for CD34 antigen"Journal of Oral Pathology and Oral Medicine. 28. 289-292 (1999)

Hiroaki Yoshida：“通过 CD34 抗原的免疫组织化学研究人颞下颌关节的血管生成”口腔病理学和口腔医学杂志。

Yoshida H, Fujita S.Yoshida T.Iizuka T, Sakakura T: "Angiogenesis in human temporomandibular joint studied by immunohistochemistry for CD34 antigen"Journal of Oral Pathology and Oral Medicine. 28. 289-292 (1999)

Yoshida H、Fujita S.Yoshida T.Iizuka T、Sakakura T：“通过 CD34 抗原的免疫组织化学研究人颞下颌关节的血管生成”口腔病理学和口腔医学杂志。