Molecular biological study on functional non-cariogenic tri-saccharide metabolized by microorganisms in the intestines

1.生长情况：齿状芽孢杆菌ATCC 17982利用少量半乳糖。牙杆菌ATCC 17982对LF的利用高于半乳糖，但不及葡萄糖、蔗糖、乳糖和果糖。口腔假丝酵母ATCC 10557利用了本研究中使用的所有碳水化合物，但半乳糖和乳糖的利用速度慢于研究中使用的其他糖。2.降解模式：在TTY-LF中检测到并积累了半乳糖，而在TTY-LF培养过程中检测到微量的其他单糖和双糖。TTY-Glac-Gal-Fru和TTY-Suc-Lac的培养上清液中仍有半乳糖存在。β-半乳糖苷酶：β-半乳糖苷酶活性最高值为0.22mmol.μ/μ/mg，在添加低聚铁后3h即可检测到，而10557则在30min时检测到0.486 mmol.min./mg。

1.Growth : A. odontolyticus ATCC 17982 utilized galactose a little. A. odontolyticus ATCC 17982 utilized LF more than galactose, but not as much as glucose, sucrose, lactose and fructose. S.oralis ATCC 10557 utilized all carbohydrate used in this study, but the rate of utilization of galactose and LF were slower than other sugars used in this study.2.Degradation pattern : Galactose was detected and accumulated in the supernatant of the medium while trace amount of the other mono-and di-saccharides were detected when A.odontolyticus ATCC 17982 and S.oralis ATCC 10557 had been grown in TTY-LF. Galactose had remained in the supernatant of the medium of TTY-Glac-Gal-Fru and TTY-Suc-Lac. S.oralis ATCC 10557 degraded the sugars more rapidly than A.odontolyticus ATCC 17982.3.β-Galactosidase : The maximum activities of β-galactosidase of A.odontolyticus ATCC 17982 was 0.22 μmol/min/mg, which was detected in 3 hours after addition of LF although those of S.oralis ATCC 10557 was 0.486 μmol/min/mg, which was detected whthin 30 min.