Molecular biological study on functional non-cariogenic tri-saccharide metabolized by microorganisms in the intestines

肠道微生物代谢功能性非致龋三糖的分子生物学研究

• 批准号: 13672137
• 负责人: HATA Shinji
• 金额: $ 2.75万
• 依托单位: Tohoku University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2001
• 资助国家: 日本
• 起止时间: 2001 至 2003
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-13672137/
• 关键词: LACTOSUCROSE; ORAL BACTERIA; ACTINOMYSES

1.Growth : A. odontolyticus ATCC 17982 utilized galactose a little. A. odontolyticus ATCC 17982 utilized LF more than galactose, but not as much as glucose, sucrose, lactose and fructose. S.oralis ATCC 10557 utilized all carbohydrate used in this study, but the rate of utilization of galactose and LF were slower than other sugars used in this study.2.Degradation pattern : Galactose was detected and accumulated in the supernatant of the medium while trace amount of the other mono-and di-saccharides were detected when A.odontolyticus ATCC 17982 and S.oralis ATCC 10557 had been grown in TTY-LF. Galactose had remained in the supernatant of the medium of TTY-Glac-Gal-Fru and TTY-Suc-Lac. S.oralis ATCC 10557 degraded the sugars more rapidly than A.odontolyticus ATCC 17982.3.β-Galactosidase : The maximum activities of β-galactosidase of A.odontolyticus ATCC 17982 was 0.22 μmol/min/mg, which was detected in 3 hours after addition of LF although those of S.oralis ATCC 10557 was 0.486 μmol/min/mg, which was detected whthin 30 min.

1.生长：A.解齿菌ATCC 17982对半乳糖利用较少。A.解齿菌ATCC 17982对LF的利用率高于半乳糖，但低于葡萄糖、蔗糖、乳糖和果糖。口腔链球菌ATCC 10557对本研究中使用的所有碳水化合物都有利用，但对半乳糖和LF的利用速度比本研究中使用的其他糖慢。2.降解模式：半乳糖被检测到并积累在培养基的上清液中，而痕量的其它单-和二-半乳糖被检测到并积累在培养基的上清液中。当溶牙放线菌ATCC 17982和口腔链球菌ATCC 10557在TTY-LF中生长时，检测到细菌。半乳糖保留在TTY-Glac-Gal-Fru和TTY-Suc-Lac培养基的上清液中。3.β-半乳糖苷酶：解齿放线菌ATCC 17982的β-半乳糖苷酶最大酶活为0.22 μmol/min/mg，而口腔链球菌ATCC 10557的β-半乳糖苷酶最大酶活为0.486 μmol/min/mg，在加入LF后3 h才检测到。

项目成果

S.Hata, H.Mayanagi: "Acid diffusion through extracellular polysaccharides produced by various mutants of Streptococcus mutans"Archives of Oral Biology. 48. 431-438 (2003)

S.Hata，H.Mayanagi：“通过变形链球菌各种突变体产生的胞外多糖进行酸扩散”口腔生物学档案。