Effects of aging on IL-1β and PGE_2 production in the periodontal ligament cells with mechanical stress.

衰老对机械应力牙周膜细胞 IL-1β 和 PGE_2 产生的影响。

• 批准号: 13672171
• 负责人: GOSEKI Takemi
• 金额: $ 2.24万
• 依托单位: Nihon University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2001
• 资助国家: 日本
• 起止时间: 2001 至 2003
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-13672171/
• 关键词: aging; Mechanical stress; Periodontal ligament cells; ヒト歯根膜細胞; メニカルストレス; NF-kB

Although the severity of periodontal disease is known to be affected by the age of the host, the pathological role of aging in periodontal disease, especially that attributable to trauma from occlusion, has not been well characterized. Prostaglandin (PG) E_2 and interleukin (IL)-1β are key mediators involved in periodontal diseases, potent stimulators of bone resorption, and are produced by human periodontal ligament (PDL) cells in response to mechanical stress. To investigate age-related changes in the biosynthetic capacity of PGE_2 and IL-1β in PDL cells, we examined the effects of in vivo aging with mechanical tension on PGE_2 and IL-1β expression by rat PDL cells. PDL cells obtained from the incisors of 6-week (young) and 60-week (old) rats were cultured on flexible-bottomed culture plates. The cells were deformed by causing a 9% or 18% increase in surface area at 6 cycles per minute for 1 to 5 days. We found an approximately twofold increase in PGE_2 and IL-1b production by old PDL cells subjected to mechanical tension compared with that by young cells, although the constitutive levels were similar in both. The expression of cyclooxygenase (COX)-2 and IL-1β mRNA was enhanced by mechanical tension as determined by use of reverse transcription-polymerase chain reaction (RT-PCR), whereas COX-1 and IL-1β-converting enzyme mRNA remained unchanged. It is possible that the large amount of PGE\2 and IL-1β produced by PDL cells from an aged host in response to mechanical force may be positively related to the acceleration of alveolar bone resorption.

虽然牙周病的严重程度是已知的主机的年龄的影响，衰老在牙周病的病理作用，特别是由于创伤从咬合，还没有得到很好的表征。前列腺素E2（Prostaglandin，PG）和白细胞介素1 β（interleukin，IL-1 β）是牙周病中的重要介质，是骨吸收的强刺激因子，由人牙周膜细胞（periodontal ligament，PDL）在机械应力作用下产生。为探讨牙周膜细胞合成PGE_2和IL-1β能力的增龄性变化，本实验观察了在体机械牵张老化对大鼠牙周膜细胞PGE_2和IL-1β表达的影响。将从6周（年轻）和60周（年老）大鼠的切牙获得的PDL细胞在柔性底培养板上培养。通过以每分钟6个循环使表面积增加9%或18%，持续1至5天，使细胞变形。我们发现老年PDL细胞在机械张力作用下产生的PGE_2和IL-1b比年轻细胞增加约2倍，尽管两者的组成性水平相似。逆转录-聚合酶链反应（RT-PCR）检测结果显示，机械牵张可增加环氧化酶（考克斯）-2和IL-1β mRNA的表达，而考克斯-1和IL-1β转换酶mRNA的表达无明显变化。老年人牙周膜细胞在机械力作用下产生大量PGE 2和IL 1β可能与牙槽骨吸收加速有关。

项目成果

山口 大 他: "ヒト歯根膜細胞のリソソーム酵素カテプシンBおよびL活性の動態"第60回日本矯正歯科学会学術大会. 196 (2001)

Dai Yamaguchi 等人：“人牙周膜细胞中溶酶体酶组织蛋白酶 B 和 L 活性的动态”，日本正畸学会第 60 届年会 196（2001 年）。

山口 大 他: "矯正治療による重度歯根吸収者由来歯髄細胞におけるneuropeptideによる炎症性サイトカイン産生能"第61回日本矯正歯科学会学術大会. 174 (2002)

Dai Yamaguchi等人：“正畸治疗后牙根严重吸收患者的牙髓细胞中神经肽产生炎症细胞因子的能力”第61届日本正畸学会学术会议174（2002年）。

Shimizu N., et al.: "Stimulation of prostaglandin E_2 and Interleukin-1β production from old rat periodontal ligament cells subjected to mechanical stress."J Gerontology. 55A. B489-B495 (2000)

Shimizu N. 等人：“受到机械应力的老年大鼠牙周膜细胞产生前列腺素 E_2 和白细胞介素 1β 的刺激”，J Gerontology 55A。

Yamaguchi M., et al.: "Effects of neuropeptides on human pulp cells obtained from orthodontic patients' with severe root resorption."The Japanese Orthodontic Society-61^<th> Annual Meeting. 174 (2002)

Yamaguchi M., et al.：“神经肽对从患有严重牙根吸收的正畸患者获得的人牙髓细胞的影响。”日本正畸学会第 61 届年会。

Simizu N., et al.: "Stimulation of prostaglandin E_2 and Interleukin-1β production from old rat periodontal ligament cells subjected to mechanical stress."J Gerontology. 55A. B489-B495 (2000)

Simizu N. 等人：“刺激老年大鼠牙周膜细胞产生前列腺素 E_2 和白细胞介素 1β”。J Gerontology 55A（2000 年）。