Evaluation of periodontal disease activity by analyzing the production of cytokines and the expression of growth factors in inflamed periodontal issues.

通过分析发炎牙周问题中细胞因子的产生和生长因子的表达来评估牙周病活动。

• 批准号: 13672191
• 负责人: HAMACHI Takafumi
• 金额: $ 1.98万
• 依托单位: Kyushu University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2001
• 资助国家: 日本
• 起止时间: 2001 至 2002
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-13672191/
• 关键词: chronic periodontitis; aggressive periodontitis; periodontal pathogens; PCR method; P.gingivalis; fimA genotype; chemokine receptor; 歯周炎; サイトカイン; 組織再生修復能

The aim of this research was to investigate the relationship between the prevalence of periodontal pathogens and the host response and the tissue regenerative activity in inflamed periodontal tissues in patients with adult chronic periodontitis and aggressive periodontitis. The detection of A.actinomycetemcomitans, P.gingivalis, B.forsythus and the determination of finA genotypes of P.gingivalis in subgingival plaque were analyzed by polymerase chain reaction methods. In aggressive periodontitis, the most prevalent fimA genotype of P.gingivalis was type II, followed by type I and the combination of type I and II. The cell number of P.gingivalis was much greater in type I positive sites than in type II positive sites. The detection frequency of 3 periodontal pathogens was higher in type II positive sites. These results suggest that P.gingivalis possessing type II fimA is associated with aggressive periodontitis in combination with other periodontal pathogens and the quantitative increase of P.gingivalis possessing type I fimA is also related to the pathogenicity. The expression of chemokine receptor mRNA and growth factor mRNA, such as basic fibroblast growth factor (bFGF) and transforming growth factor-β(TGF-β), were examined by RT-PCR method in inflamed periodontal tissues. CXCR1&2, CCR3 and CCR4 mRNA were highly expressed, while low expression was observed with CXCR3 and CCR5. The expression of TGF-β mRNA was very few, however, the expression of bFGF was universally detected in inflamed periodontal tissues. These results suggest that high proportion of CCR3 and CCR-4-positive T lymphocytes, indicative of Th2 lymphocyte, may play an important role in the inflamed periodontal tissues.

本研究的目的是探讨成人慢性牙周炎和侵袭性牙周炎患者牙周病原体的流行与宿主反应和炎症牙周组织组织再生活性之间的关系。采用聚合酶链反应（PCR）方法检测龈下菌斑中伴放线放线菌、牙龈卟啉单胞菌、牙龈B螺旋体的检出率及牙龈卟啉单胞菌的finA基因型。在侵袭性牙周炎中，牙龈卟啉单胞菌fimA基因型以II型最多见，其次为I型和I型与II型的混合型。牙龈卟啉单胞菌Ⅰ型阳性部位的细胞数明显多于Ⅱ型阳性部位。3种牙周致病菌在Ⅱ型阳性部位检出率较高。这些结果表明，具有II型fimA的牙龈卟啉单胞菌与其他牙周病原体的结合与侵袭性牙周炎有关，并且具有I型fimA的牙龈卟啉单胞菌的数量增加也与致病性有关。采用RT-PCR方法检测炎症牙周组织中趋化因子受体mRNA和生长因子碱性成纤维细胞生长因子（bFGF）、转化生长因子-β（TGF-β）mRNA的表达。CXCR 1和2、CCR 3和CCR 4 mRNA高表达，而CXCR 3和CCR 5 mRNA低表达。TGF-β mRNA在炎症牙周组织中的表达很少，而bFGF在炎症牙周组织中普遍表达。结果提示，高比例的CCR-3和CCR-4阳性T淋巴细胞（Th 2淋巴细胞）可能在牙周炎中起重要作用。