Evaluation of the periodontal disease activity by the quantitative analysis of periodontal pathogens using polymerase chain reaction method

放线菌、牙龈卟啉单胞菌和连翘拟杆菌被认为是牙周病的病原生物。本研究项目的目的是利用基于聚合酶链反应（PCR）的方法来检测和定量这些牙周病病原体，并检查这些病原体单独或联合存在与初始牙周治疗效果之间的关系，以评估疾病的活动性。在基线和初始治疗后，从中度牙周炎患者的牙周袋中收集牙龈下菌斑样本。以探诊出血（BOP）作为临床指标。在这三种细菌共存的牙周部位，以及放线菌双歧杆菌与牙龈假单胞菌或连翘假单胞菌共存的部位，初始治疗后BOP仍有维持的趋势。随着放线菌胞数在基线时的增加，BOP也趋于在初始治疗后保持不变。基线时放线菌细胞数量的增加与初始治疗后同时检测三种细菌的频率密切相关。初始治疗后，在这三种细菌共存的部位观察到相当频繁的BOP。这些结果表明，基线时放线菌A.的定量以及牙龈P.和连翘B.的检测可用于预测初始治疗的效果和了解牙周病的活动性。

Actinobacillus actinomycetemcomitans, Porphyromonas gingivalis and Bacteroides forsythus have been considered as causative organisms of periodontal disease. The aim of this research project was to evaluate the disease activity by using a polymerase chain reaction (PCR) based method for detection and quantification of these periodontopathogens and examine the relationship between the presence of these pathogens alone or in combination and the effect of the initial periodontal therapy. Subgingival plaque samples were collected from the periodontal pockets of patients with moderate periodontitis at baseline and after initial therapy. The bleeding on probing (BOP) was chosen as clinical parameter. In the periodontal site where these three bacteria co-exsist and n the site where A. actinomycetemcomitans exists with either P. gingivalis or B. forsythus, BOP trends to remain after the initial therapy. In proportion as the number of A. actinomycetemcomitans cells increases at baseline, BOP also trends to remain after the initial therapy. The increase of the number of A. actinomycetemcomitans cells at baseline is strongly related to the frequency of simultaneous detection three bacteria after the initial therapy. BOP after the initial therapy were observed with considerable frequency in the site where these three bacteria co-exsist. These results suggest that the quantification of A. actinomycetemcomitans at baseline and the detction of P. gingivalis and B. forsythus are available to predict the effect of the initial therapy and comprehend the periodontal disease activity.