Characteristics of flurothyl-induced seizures and the evaluation of the model of development of epilepsy

1)在氟醚点燃模型中，8次氟醚诱导的全身性阵挛发作（称为前脑发作）以及随后的28天无刺激间隔将小鼠的发作表型从纯粹的前脑发作改变为更复杂的发作表型。当氟醚再次发作时，全面性前脑发作迅速发展为具有强直性表现的发作，称为脑干发作。2)氟醚试验前每天给药丙戊酸盐、唑尼沙胺和苯妥英8 d，丙戊酸盐和唑尼沙胺显著抑制小鼠癫痫发作表型从慢性发作到强直性发作的变化，而苯妥英则无此作用。3) MK-801对初代小鼠和氟醚点燃小鼠的急性作用中，对慢性癫痫发作的潜伏期没有影响。然而，MK-801剂量依赖性地抑制小鼠强直性癫痫发作的潜伏期和发生率，并抑制氟醚点燃小鼠癫痫发作严重程度的等级。此外，反复给药MK-801对阵挛发作的潜伏期没有任何影响。然而，当小鼠在最后一次给药后11周再次暴露于不含mk - 8011的氟醚时，癫痫发作严重程度明显加重。4)蒙古沙鼠在出现阵挛性惊厥后1 min内立即出现强直伸展。在出现阵挛性惊厥的同时，皮层、海马和杏仁核出现了非常高振幅的尖峰波。5) 6-OHDA-和spd -4给药小鼠的癫痫模式没有改变。这一发现与电引燃模型完全不同。

1) In the flurothyl kindling model, eight flurothyl-induced generalized clonic seizures, dubbed forebrain seizures, and a subsequent 28-days stimulation-free interval changed the seizure phenotype of mice from purely forebrain seizure to seizures of a more complex phenotype. The generalized-onset, forebrain seizure progresses rapidly to a seizure with tonic manifestations, dubbed brain-stem seizures, when re-challenged with flurothyl.2) When valproate, zonisamide and phenytoin were administered to mice daily for 8 d prior to the flurothyl trial changes in the seizure phenotype from clonic to tonic seizure were significantly inhibited by valproate and zonisamide, but not by phenytoin.3) In the acute effect of MK-801 in naive and flurothyl-kindled mice, there were no changes on the latencies of clonic seizures. However, MK-801 dose-dependently inhibited both latencies and the incidence of tonic seizures in mice and suppressed the grade of seizure severity in flurothyl-kindled mice. In addition, repeated administration of MK-801 did not have any effect on the latencies of clonic seizure. However, seizure severity was significantly exacerbated when mice were re-exposed to flurothyl without MK-801 1 week after the last administration.4) Mongolian gerbils demonstrated tonic extension immediately after within 1 min after the appearance of clonic convulsion. Very high amplitude spike waves appeared in the cortex, hippocampus and amygdala concurrent with the appearance of clonic convulsion.5) The epilepsy pattern was not changed in 6-OHDA- and DSP-4-administered mice. This finding is completely different with electrical kindling model.