Effects of intake of K-containing food among passive-smoking pregnant women on prevention of neonatal bleeding

被动吸烟孕妇摄入含钾食物对预防新生儿出血的影响

• 批准号: 13672525
• 负责人: NAGAMI Keiko
• 金额: $ 0.77万
• 依托单位: Mie Prefectural College of Nursing
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2001
• 资助国家: 日本
• 起止时间: 2001 至 2002
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-13672525/
• 关键词: vitamin K; cord blood; vitamin K-dependent clotting factor; passive smoking; neonate

Study objective : The objectives of the present study were to elucidate the effects of passive smoking during pregnancy on changes in vitamin K-dependent clotting factor activity in cord blood, and to determine the effects of the intake of vitamin K-containing food among passive smoking pregnant women on prevention of neonatal bleeding, from the perspective of the transplacental supply of vitamin K.Study methods : Subjects were 148 non-smoking women who underwent vaginal delivery (passive smoking group, n=53; non-passive smoking group, n=95). Consent was obtained following written and oral explanations. Following delivery, citric acid-added plasma obtained from collection of umbilical venous blood was used to investigate the following : prothrombin time, activated partial thromboplastin time, hepaplastin test, thrombotest, vitamin K-dependent clotting factor activity (FII, FVII, FIX, FX) and non-vitamin K-dependent clotting factor activity. In addition, a method for measuring vitamin K … More in cord blood was investigated based on Wakabayashi et al.(Folia Pharmacol.Jpn.116)'s HPLC using platinum catalyst reduction and electrochemical detection.Results and discussion : The passive smoking group had significantly lower FVII and FIX than the non-passive smoking group. Comparison of the two groups with regard to the intake of fermented soybeans revealed a significantly higher FVII in the non-passive smoking group relative to the passive smoking group in the non-intake group, with no significant intergroup differences in the intake group. Comparison of the two groups with regard to the intake of broccoli revealed a significantly higher thrombotest value in the non-passive smoking group relative to the passive smoking group in the non-intake group, with no significant intergroup differences in the intake group. For pregnant women who may have difficulty avoiding passive smoking, regular intake of vitamin K-containing food may improve the activity of vitamin K-dependent clotting factors in fetal blood and thus provide a means of preventing early neonatal bleeding. Less

研究目的：本研究旨在阐明孕期被动吸烟对脐带血中维生素k依赖性凝血因子活性变化的影响，并从经胎盘提供维生素k的角度探讨被动吸烟孕妇摄入含维生素k的食物对预防新生儿出血的影响。研究对象为148例经阴道分娩的非吸烟妇女（被动吸烟组53例，非被动吸烟组95例）。经书面和口头解释后获得同意。分娩后，采集脐静脉血中添加柠檬酸的血浆，检测以下指标：凝血酶原时间、活化的部分凝血活素时间、肝活素试验、凝血试验、维生素k依赖性凝血因子活性（FII、FVII、FIX、FX）和非维生素k依赖性凝血因子活性。此外，在Wakabayashi等人（Folia Pharmacol.Jpn）的基础上，研究了脐带血中维生素K…More的测定方法。116)采用铂催化剂还原和电化学检测的高效液相色谱法。结果与讨论：被动吸烟组FVII和FIX明显低于非被动吸烟组。比较两组对发酵大豆的摄入量，发现非被动吸烟组的FVII明显高于非被动吸烟组，而摄入组间无显著差异。两组在西兰花摄入量方面的比较显示，非被动吸烟组的血栓测试值明显高于非被动吸烟组，而摄入组的组间差异不显著。对于那些可能难以避免被动吸烟的孕妇，定期摄入含维生素k的食物可能会提高胎儿血液中依赖维生素k的凝血因子的活性，从而提供一种预防新生儿早期出血的方法。少