Effects of endurance training with hypoxic stimulation induced by Co^<2+> treatment on oxygen transport system in rat skeletal muscles

Co^2处理低氧刺激耐力训练对大鼠骨骼肌氧运输系统的影响

• 批准号: 13680011
• 负责人: SUZUKI Junichi
• 金额: $ 1.92万
• 依托单位: Hokkaido University of Education
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2001
• 资助国家: 日本
• 起止时间: 2001 至 2002
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-13680011/
• 关键词: angiogenesis; training; hypoxia; cobalt; enzyme activity; skeletal muscle; endurance capacity; 酸素活性

This study was designed to examine the changes in the capillary geometry and expression of angiogenic growth factor in skeletal muscles after endurance training with and without chronic CoCl_2 administration. The Co^<2+>-treated rats drank water containing 0.01% CoCl_2. In the first experiment, training and Co^<2+> administration lasted for 5 weeks. Chronic Co^<2+> administration significantly increased the blood hemoglobin concentration by approximately 25% with and without training. Only in the diaphragm, the Co^<2+> treatment alone significantly increased total capillary density and the capillary-to-fiber ratio (C:F). Both training groups with and without Co^<2+> administration significantly increased the C:F in soleus (SOL) and plantaris (PL) muscles. In PL, the increase was significantly greater in the Co^<2+>-treated training group than in the non-Co^<2+>-treated training group. The densities of VEGF-positive capillaries (VEGF-cap) remained unchanged in all muscle portions examin … More ed after either Co^<2+> administration or exercise training. The second experiment was designed to examine the time course changes in VEGF expression and capillary geometry in the early stage of the treatment. After 6 and 10 days of the Co^<2+> administration, the VEGF-cap was significantly increased by 27% and 65%, respectively in SOL. After 10 days of Co^<2+> administration, the C:F was significantly increased. The training with Co^<2+> significantly increased the VEGF-cap by 69%, 44% and 60%, respectively, after 3, 6 and 10 days in SOL, while it significantly increased the VEGF-cap after 10 days in the mixed fiber portion of plantaris muscle (PLm). Training with or without Co^<2+> significantly increased the C:F after 6 and 10 days in the SOL. Training with Co^<2+>, but training alone did not, significantly increased the C:F in PLm after 10 days. In condition, the present results suggest that activation of the cellular oxygen-sensing mechanism induced by Co^<2+> administration facilitates microvascular remodeling in hind-leg muscles by facilitating an expression of VEGF. Less

本研究旨在观察耐力训练前后骨骼肌毛细血管几何形态及血管生成生长因子表达的变化。Co^<2+>处理的大鼠饮用含有0.01% CoCl_2的水。第1组训练和Co^<2+>给药5周。慢性Co^<2+>管理显著提高血液血红蛋白浓度约25%，有无训练。仅在横膈膜，单独Co^<2+>处理显著增加了总毛细血管密度和毛细血管-纤维比（C:F）。无论是否给予Co^<2+>的训练组，比目鱼肌（SOL）和跖肌（PL）的C:F均显著增加。在PL中，Co^<2+>处理的训练组的增加明显大于非Co^<2+>处理的训练组。在所有检查的肌肉部位，vegf阳性毛细血管密度（VEGF-cap）保持不变，在Co^<2+>给药或运动训练后更明显。第二个实验旨在检测治疗早期VEGF表达和毛细血管几何形状的时间变化。Co^<2+>给药6天和10天后，SOL中VEGF-cap分别显著增加27%和65%。Co^<2+>给药10天后，C:F显著增加。Co^<2+>的训练在SOL训练3、6和10天后，VEGF-cap分别显著增加69%、44%和60%，而在10天后，planplans肌肉混合纤维部分（PLm）的VEGF-cap显著增加。无论是否进行Co^<2+>的训练，均显著提高了SOL 6天和10天后的C:F。Co^<2+>的训练，但单独训练没有显著提高PLm 10天后的C:F。在这种情况下，本研究结果表明，Co^<2+>诱导的细胞氧感应机制的激活通过促进VEGF的表达促进了后腿肌肉微血管重构。少

项目成果

Junichi SUZUKI: "Changes in arteriolar and venular portions of capillary after endurance exercise training with low concentration Co^<2+> treatment in rats"Advances in Exercise and Sports Physiology. 7巻4号. 186 (2001)

Junichi SUZUKI：“用低浓度Co^2+治疗大鼠的耐力运动训练后毛细血管的小动脉和心室部分的变化”运动和运动生理学进展第7卷，第4.186期(2001)。

Suzuki J.: "Changes in arteriolar and venular portions of capillary after endurance training with low concentration Co^<2+> treatment in rats"Advances in Exercise and Sports Physiology. 7(4). 186 (2001)

Suzuki J.：“用低浓度Co^2治疗大鼠耐力训练后毛细血管小动脉和小静脉部分的变化”运动和运动生理学进展。