Gene expressions of IGP-I, FGFs, and MyoD following denervation and compensatory overload.

去神经和代偿性超负荷后 IGP-I、FGF 和 MyoD 的基因表达。

• 批准号: 13680044
• 负责人: YAMAGUCHI Akihiko
• 金额: $ 1.09万
• 依托单位: Health Sciences University of Hokkaido
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2001
• 资助国家: 日本
• 起止时间: 2001 至 2002
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-13680044/
• 关键词: compensatory overload; muscle hypertrophy; myosin heavy chain isoforms; IGF-I; FGF; MyoD

This study was undertaken to investigate the gene expressions of IGF-I, FGFs and MyoD following denervation and compensatory overload. 10 wk-olds Wistar strain rats were used in this study. The rats were divided into 4 groups, innervated and compensatory overloaded group (NM-OL), innervated and sham operated group (NM-Cont), denervated and compensatory overloaded group (DN-OL), and denervated and sham operated group (DN-Cont). IGF-I, PGFs and MyoD mRNA expressions as well as weights of plantaris muscle were measured 3, 7 and 21 days, and myosin heavy chain isoforms were measured 21 days after the treatments. At 21 days, type I myosin heavy chain isoform in NM-OL and DN-OL were significantly increased as compared with NM-Cont and DN-Cont, respectively. At 3, 7 and 21 days, muscle weights of the plantaris in NM-OL and DN-OL were significantly increased as compared with NM-Cont and DN-Cont, respectively. At 3, 7 and 21 days, FGF-2 mRNA and FGF-6 mRNA expressions in NM-OL and DN-OL were increased as compared with NM-Cont and DN-Cont, respectively. At 3 and 7 days, IGF-I mRNA and MyoD mRNA expressions in NM-OL and DN-OL were increased as compared with NM-Cont and DN-Cont, respectively. The plantaris muscles were strophied by the denervation. The expressions of FGF-2 mRNA and FGF-6 mRNA in DN-Cont and DN-OL were lower than the NM-Cont and NM-OL, respectively, however, the expressions of IGF mRNA and MyoD mRNA in DN-Cont and DN-OL were higher or unchanged. Thus it would seem to be likely that IGP-I, FGFs and MyoD were completely associated with the morphological changes of plantaris muscle following the denervation and compensatory overload.

本研究旨在探讨失神经支配和代偿性超负荷后IGF-I、FGFs和MyoD的基因表达。本实验选用10周龄Wistar系大鼠。将大鼠分为4组，即神经支配代偿性超负荷组（NM-OL）、神经支配假手术组（NM-Cont）、失神经支配代偿性超负荷组（DN-OL）和失神经支配假手术组（DN-Cont）。分别于第3、7、21天测定大鼠跖肌重量及IGF-I、PGFs和MyoD mRNA表达，并于第21天测定肌球蛋白重链亚型。在21天时，与NM-Cont和DN-Cont相比，NM-OL和DN-OL中的I型肌球蛋白重链亚型分别显著增加。在第3、7和21天，与NM-Cont和DN-Cont相比，NM-OL和DN-OL组的跖肌重量分别显著增加。在第3、7和21天，与NM-Cont和DN-Cont相比，NM-OL和DN-OL中FGF-2 mRNA和FGF-6 mRNA的表达分别增加。在第3天和第7天，与NM-Cont和DN-Cont相比，NM-OL和DN-OL中IGF-I mRNA和MyoD mRNA的表达分别增加。跖肌因失神经支配而萎缩。DN-Cont和DN-OL中FGF-2和FGF-6 mRNA的表达分别低于NM-Cont和NM-OL，而IGF mRNA和MyoD mRNA的表达则高于NM-Cont和NM-OL或无变化。因此，IGP-I、FGFs和MyoD似乎很可能与去神经和补偿性超负荷后跖肌的形态变化完全相关。

项目成果

Akihiko Yamaguchi, Yasuhiro Ikeda, Toshihiro Hirai, Takahiko Fujikawa, and Isao Morita: "Local changes of IGF-I mRNAm GH receptor mRNA, and fiber size in rat plantaris muscle following compensatory overload"Japanese journal of Physiology. 53. 53-60 (2003)

Akihiko Yamaguchi、Yasuhiro Ikeda、Toshihiro Hirai、Takahiko Fujikawa 和 Isao Morita：“代偿性超负荷后大鼠跖肌中 IGF-I mRNA、GH 受体 mRNA 的局部变化和纤维尺寸”日本生理学杂志。

Akihiko Yamaguchi: "Local changes of IGF-I mRNA, GH receptor mRNA, and fiber size in rat plantaris muscle following compensatory overload"Japanese journal of Physiology. 53. 53-60 (2003)

Akihiko Yamaguchi：“代偿性超负荷后大鼠跖肌中 IGF-I mRNA、GH 受体 mRNA 和纤维大小的局部变化”日本生理学杂志。