Morphological changes and molecular mechanism in the carotid bodies after the termination of chronic hypoxia

慢性缺氧终止后颈动脉体的形态变化及分子机制

• 批准号: 13680048
• 负责人: KUSAKABE Tatsumi
• 金额: $ 1.98万
• 依托单位: Kokushikan University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2001
• 资助国家: 日本
• 起止时间: 2001 至 2002
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-13680048/
• 关键词: Carotid body; Hypoxia; Termination of hypoxia; Morphometry; Vasodilation; Neuropeptide; Immunohistochemistry; Second messenger; 頚動脈小体; 自律神経支配; ラット

The carotid bodies after 2, 4, and 8 weeks of hypoxic exposure were enlarged by 1.2-1.5 times in the short axis, and 1.3-1.7 times in the long axis in comparison with the normoxic control ones. The enlarged carotid bodies contained a number of expanded vasculatures. Mean density of substance P (SP) and calcitonin gene-related peptide (CGRP) containing fibers was transiently high in the carotid bodies after 4 weeks hypoxic exposure, and decreased in those after 8-12 weeks exposure. Density of vasoactive intestinal polypeptide (VIP) fibers increased significantly in all periods of hypoxic exposure observed, and was especially high in those after 4 weeks exposure. Density of neuropeptide Y fibers was unchanged in the carotid bodies during hypoxic exposure. These characteristic changes suggest a possibility that the role of these neuropeptide containing fibers may be different in the carotid bodies after each of four periods of hypoxic exposureIn the carotid bodies after 8 weeks of hypoxic exposure, the percentage of small vessels with diameters less than 5 μm decreased to under 20%, that of diameters greater than 16 μm increased to over 20%. The carotid bodies 1 and 2 weeks after the termination of chronic hypoxia were diminished in size, although their short and long axes remained larger than the normoxic controls. Those 4 and 8 weeks after the termination of chronic hypoxia were diminished to nearly normoxic control level. In those 8 weeks after the termination of chronic hypoxia, about 45% of the blood vessels were small ones with diameters less than 5 μm and 10 μm. The percentage of relatively large vessels with diameters greater than 16 μm was less than 10%. In the carotid bodies 4 weeks after the termination of hypoxia, the density of NPY fibers increased

缺氧2、4、8周后，颈动脉体短轴增大1.2-1.5倍，长轴增大1.3-1.7倍。增大的颈动脉体含有大量扩张的血管。低氧暴露4周后，颈动脉体内含P物质（SP）和降钙素基因相关肽（CGRP）纤维的平均密度短暂增高，8-12周后降低。血管活性肠多肽（VIP）纤维的密度增加显着在所有观察到的低氧暴露期间，尤其是在那些暴露4周后。在缺氧暴露期间，颈动脉体中的神经肽Y纤维的密度不变。这些特征性的变化提示，在4个低氧暴露期中，这些含神经肽纤维在颈动脉体中的作用可能不同。在低氧暴露8周后的颈动脉体中，直径小于5 μm的小血管所占的百分比下降到20%以下，直径大于16 μm的小血管所占的百分比增加到20%以上。颈动脉体1和2周后终止慢性缺氧的大小减少，虽然他们的短轴和长轴仍然大于常氧对照组。慢性缺氧结束后4周和8周，缺氧大鼠的脑组织已恢复到接近常氧对照组的水平。在慢性缺氧结束后8周，约45%的血管为直径小于5 μm和10 μm的小血管。直径大于16 μm的相对较大血管的百分比小于10%。缺氧结束后4周，颈动脉体内NPY纤维密度增加

项目成果

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