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BIOVAILABILITY OF PHYTOESTROGENS : RESEARCH ON FOOD FACTORS AS PROMOTERS OF ISOFLAVONE ABSORPTION AND PREVENTION OF LIFESTYLE-RELATED DISEASES

植物雌激素的生物利用度：作为异黄酮吸收促进剂和预防生活方式相关疾病的食物因素的研究

基本信息

批准号：
13680160
负责人：
UEHARA Mariko
金额：
$ 1.28万
依托单位：
TOKYO UNIVERSITY OF AGRICULTURE
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2001
资助国家：
日本
起止时间：
2001 至 2002
项目状态：
已结题

项目摘要

Soy isoflavones are structurally similar to estrogens and are therefore called phytoestrogens, and may prevent sex hormone-related cancer and osteoporosis. However, few studies regarding the effects of other chemicals on isoflavone bioavailability have been reported. Since almost all phytoestrogens in food exist as glycosides, intestinal bacteria glucosidases are required to hydrolyze the glycosidic bonds to enable intestinal absorption. Fructooligosaceharides (FOS), a mixture of indigestible and fermentable sugars, stimulate the growth of bifidobacteria in the intestine. Thus, it is postulated that dietary FOS may affect the bioavailability of phytoestrogen glycosides and therefore improve their absorption in the gut, thus having beneficial effects on lifestyle-related diseases.In 2001, the kinetics of isoflavones in rats fed a FOS supplemented diet or a control diet were examined by measuring genistein and daidzein concentrations in blood collected from three different veins, and by … More measuring urinary excretion at 24-and 48-hours after a single intragastric ministration of isoflavone conjugates. The genistein concentration in the portal blood increased rapidly, reaching a peak in both FOS and control groups at 1h after administration. The concentrations in the central venous blood were approximately half of those in the portal blood. In the FOS fed group, both genistein and daidzein remained detectable in the tail venous blood after 24-48 hours. The urinary excretion of both isoflavones at 24-and 48-hours after administration was significantly higher in the FOS-fed group than in the control gloup.In 2002, the synergistic effects of dietary isoflavone and FOS supplementation on post-gastrectomy (OX) osteopenia and anemia were determined. Decreases in femoral trabecular bone mineral density and bone breaking force by OX were inhibited in rats fed the FOS with and without isoflavone diets, whereas isoflavone only supplementation did not prevent bone loss in OX rats. Notably, a combination isoflavone and FOS diet enhanced production of equol, which has a strong estrogenic effect, from daidzein in OX rats. Moreover, both isoflavone and FOS supplementations prevented increases of hepatic lipid hydroperoxides and serum TNF-α, a marker of tissue necrosis, in post-OX anemic rats. Less

大豆异黄酮在结构上与雌激素相似，因此被称为植物雌激素，可以预防与性激素相关的癌症和骨质疏松。然而，关于其他化学物质对异黄酮生物利用度的影响的研究很少有报道。由于食物中几乎所有的植物雌激素都以糖苷的形式存在，肠道细菌需要葡萄糖苷酶来水解糖苷键以实现肠道吸收。低聚果糖(FOS)是一种不可消化和可发酵的糖的混合物，可以刺激肠道中双歧杆菌的生长。因此，推测膳食低聚果糖可能影响植物雌激素糖苷的生物利用度，从而改善它们在肠道中的吸收，从而对生活方式相关疾病有有益的影响。2001年，通过测定三种不同静脉采集的血液中染料木素和大豆苷元的浓度以及…法，研究了饲喂低聚果糖饮食或对照饮食的大鼠体内异黄酮类成分的动力学进一步测量单次灌胃异黄酮偶联物后24小时和48小时的尿液排泄量。门静脉血中金雀异黄素浓度迅速升高，FOS组和对照组在给药后1h均达高峰。在中心静脉血中的浓度约为门静脉血浓度的一半。在果寡糖饲喂组，24-48小时后，金雀异黄素和大豆苷元仍可在尾静脉血中检测到。在给药后24小时和48小时，饲喂低聚果糖组大鼠尿中两种异黄酮类成分的排泄量均显著高于对照组。补充异黄酮组和单纯补充异黄酮组大鼠股骨松质骨密度和破骨力的下降均受到抑制，而单纯补充异黄酮组不能阻止OX大鼠的骨丢失。值得注意的是，异黄酮加FOS饮食增加了黄牛大鼠体内大豆苷元产生的马酚，这种马酚具有很强的雌激素样作用。此外，补充异黄酮类和低聚果糖均可防止氧化应激后大鼠肝脏过氧化脂质和血清肿瘤坏死因子-α的升高，肿瘤坏死因子是组织坏死的标志。较少