Deterioration action by solar ultraviolet rays exposure on infection and symptom of Schistosomiasis and in symptom, and evaluation it

太阳紫外线照射对血吸虫病感染及症状的恶化作用及其评价

• 批准号: 13680630
• 负责人: OHWATARI Nobu
• 金额: $ 2.37万
• 依托单位: Nagasaki University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2001
• 资助国家: 日本
• 起止时间: 2001 至 2002
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-13680630/
• 关键词: Solar ultraviolet rays; Depression of immunity; Sick infection; Schistosomiasis mansoni; DDY mouse; Evaluation of UV influence; 紫外線; マンソン住血吸虫

On this study, we experimented about a change of immunological ability induced by exposure of solar ultraviolet rays and an influence of the change on a sick infection, and examine whether ultraviolet rays participates in sick infection, or not. We used cercariae of Schistosoma mansoni for infectious experiments.In results of artificial ultraviolet rays (UV-B) irradiation with 400 J/m^2 of UV-B, Schistosomiasis became worsen by UV-B irradiation, because both cercariae invaded into skin tissue and adult warm recovered increased significantly as comparison with non-irradiation group (p<0.01).In solar light exposure contained with 400 J/m^2 of UV-B, there was not significant difference in numbers of each cercariae invaded into skin tissue and adult warm recovered as comparison with non-exposure group (control group).In solar light irradiation contained with 800 J/m^2 of UV-B, the body temperature of mice increased by infrared rays, so we used with ice pack for restrain it by behavior ther … More mo-regulation. On immunity, CD4/CD8 in mice decreased to the significant minimum value in 7 days after solar light exposure, but it recovered in after 2 weeks. The cercariae invaded increased significantly compared with control group (p<0.05). However, it was not significant difference in adult warm recovered.Further more in solar light exposure with 1600 J/m^2, we stopped infection experiments because damages induced by sunstroke in mice.As considerations, the effective radiation dose of UV-B in solar light was high compared with artificial UV-B. It is guessed that continuous spectrum of solar light need much dose to get equal effects compared with discontinuity spectra of artificial UV-B.Exposure to solar light at 800 J/m^2 of UV-B caused depression of immunity, and it enhanced cercariae to invade into skin tissue. These suggest that UV-B in solar light becomes worsen in infection of Schistosomiasis. Furthermore, wemust investigate for human health about growing up of cercariae in the body with solar light exposure through experiments over 800 J/m^2 with temperature control equipment. Less

在这项研究中，我们实验了暴露于太阳紫外线引起的免疫能力的变化以及这种变化对疾病感染的影响，并检查紫外线是否参与了疾病感染。本文以曼氏血吸虫尾蚴为研究对象，用400J/m~2的UV-B进行人工紫外线照射，与未照射组比较，两者侵入皮肤组织和成虫温热恢复率均显著增加(p&lt；在400J/m~2的UV-B的太阳光照射下，各次尾蚴侵入皮肤组织的数量和成人体温的恢复与未暴露组(对照组)相比无显著差异。在含800J/m~2的UV-B的太阳光照射下，小鼠的体温因红外线而升高，因此我们用冰袋来抑制其对…的行为更多的监管。免疫方面，小鼠在太阳光照射后7天，CD_4/CD_8降至最低值，2周后恢复正常。尾蚴侵袭量较对照组显著增加(p&lt；0.05)。此外，在1600J/mm2的太阳光照射下，由于中暑对小鼠造成损伤，我们停止了感染实验。考虑到太阳光中UV-B的有效辐射剂量高于人工UV-B。推测太阳光的连续光谱需要更大的剂量才能达到与人工UV-B间断光谱相同的效果。暴露在800J/m~2的UV-B太阳光下会导致免疫抑制，并促进尾蚴侵入皮肤组织。这表明，在血吸虫病感染过程中，太阳光中的UV-B会变得更加严重。此外，我们还将利用温控装置，通过800J/m~2以上的实验，研究人体尾蚴在日光照射下的生长情况。较少

项目成果

6. Ohwatari N, Kosaka M, Pleschka K & Simon E, Eds. Kosaka M, Sugahara T, Schmidt K L & Simon E: "Protective measures against solar radiant heat and ultraviolet. Thermotherapy for Neoplasia, Inflammation, and Pain, pp.88-94"Springer-Verlag. 500 (2001)

6. 大渡 N、小坂 M、普莱施卡 K

Jutavijittum P., et al.: "Present epidemiological pattern of antibody to hepatitis a virus among Chiang Mai children, Northern Thailand"Asian J Trop Med Public Health. 33(2). 268-271 (2002)

Jutavijittum P. 等人：“泰国北部清迈儿童中甲型肝炎病毒抗体的流行病学模式”亚洲 J Trop Med 公共卫生杂志。

Kumatori A, Yang D, Suzuki S, Nakamura M: "Cooperation of STAT-1 and IRF-1 in interferon-gamma-induced transcription of the gp91 (phox) gene"J Biol Chem.. 277(11). 9103-9111 (2002)

Kumatori A、Yang D、Suzuki S、Nakamura M：“STAT-1 和 IRF-1 在干扰素-γ 诱导的 gp91 (phox) 基因转录中的合作”J Biol Chem.. 277(11)。

Parquet M.C., et al.: "St.Louis encephalitis virus induced pathology in cultured cells"Arch Virol.. 147(6). 1105-1119 (2002)

Parquet M.C. 等人：“圣路易斯脑炎病毒在培养细胞中诱导病理学”Arch Virol.. 147(6)。

Itoh M, Weerasooriya MV, Qiu G, Gunawardena NK, Anantaphruti MT, Tesana S, Rattanaxay P, Fujimaki Y, Kimura E.: "Sensitive and specific enzyme-linked immunosorbent assay for the diagnosis of Wuchereria bancrofti infection in urine samples"Am J Trop Med Hy

Itoh M、Weerasooriya MV、Qiu G、Gunawardena NK、Anantaphruti MT、Tesana S、Rattanaxay P、Fujimaki Y、Kimura E.：“用于诊断尿液样本中班氏菌感染的灵敏和特异性酶联免疫吸附测定”Am J