ANALYSIS OF CHROMOSOMESEGREGATION MECHANISM BY HUMAN CH-TOG RELATED GENES IN YEAST

人CH-TOG相关基因在酵母中的染色体分离机制分析

• 批准号: 13680779
• 负责人: NAKASEKO Yukinobu
• 金额: $ 2.24万
• 依托单位: KYOTO UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2001
• 资助国家: 日本
• 起止时间: 2001 至 2003
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-13680779/
• 关键词: KINETOCHORE; CHROMOSOME; CANCER CELL; CHROMOSOME SEGREGATION; YEAST

Fission yeast S. pombe dis1 mutants are defective in sister chromatids separation. Dis1 belongs to an evolutionarily conserved family of microtubule binding proteins, including human ch-TOG, frog XMAP215, fly minispindles and budding yeast Stu2p. We found Dis1-GFP signals are associated with cytoplasmic microtubules, spindle microtubules and spindle pole bodies. Surprisingly, as cells progress from metaphase to anaphase during mitosis, Dis1-GFP signals mimic those of kinetochore proteins such as Mis6, Mis12. Dis1 is the first fission yeast protein that associates with both microtubules and the centromeric DNA of the kinetochore. Dis1 may play a dual role by becoming a part of the kinetochores in an M-phase specific manner, possibly generating connections between kinetochores and microtubules.We have also shown that protein highly related to Dis1, called Mtc1/Alp14, behaves in a similar but not identical manner to Dis1. Their behavior suggests that these two related molecules have significant differences in interaction with microtubules.

裂殖酵母粟酒裂殖酵母dis 1突变体在姐妹染色单体分离中有缺陷。Dis 1属于进化上保守的微管结合蛋白家族，包括人ch-TOG、青蛙XMAP 215、苍蝇小孢子和芽殖酵母Stu 2 p。我们发现Dis 1-GFP信号与细胞质微管、纺锤体微管和纺锤体极体有关。令人惊讶的是，随着细胞在有丝分裂过程中从中期进展到后期，Dis 1-GFP信号模拟了诸如Mis 6、Mis 12等动粒蛋白的信号。Dis 1是第一个与微管和着丝粒DNA结合的裂殖酵母蛋白。Dis 1可能发挥双重作用，以M期特异的方式成为着丝粒的一部分，可能产生着丝粒和微管之间的连接。我们还发现，与Dis 1高度相关的蛋白质，称为Mtc 1/Alp 14，以类似但不完全相同的方式表现出与Dis 1。它们的行为表明，这两种相关分子在与微管的相互作用方面存在显著差异。

项目成果

Fukumoto, Y., Hiyama, H., Yokoi, M., Nakaseko, Y., Yanagida, M., Hanaoka, F.: "Two budding yeast RAD4 homologs in fission yeast play different roles in the repair of UV-induced DNA damage."DNA repair. 1・10. 833-845 (2002)

Fukumoto, Y.、Hiyama, H.、Yokoi, M.、Nakaseko, Y.、Yanagida, M.、Hanaoka, F.：“裂殖酵母中的两种出芽酵母 RAD4 同源物在紫外线诱导的 DNA 修复中发挥不同的作用《DNA修复》。1・10.833-845(2002)

Nakaseko, Y., Goshima, G., Morishita, J., Yanagida, M.: "M phase-specific kinetochore proteins in fission yeast : microtubule-associating Dis1 and Mtc1 display rapid separation and segregation during anaphase."Current Biology. 11. 537-549 (2001)

Nakaseko, Y.、Goshima, G.、Morishita, J.、Yanagida, M.：“裂殖酵母中的 M 期特异性着丝粒蛋白：微管相关的 Dis1 和 Mtc1 在后期表现出快速分离和分离。”当前生物学。

Nakamura, T., Nagao, K., Nakaseko, Y., Yanagida, M.: "Cut1/separase C-terminus affects spindle pole body positioning in interphase of fission yeast pointed nuclear formation."Genes to Cells. Vol.7 No.11. 1113-1124 (2002)

Nakamura, T.、Nagao, K.、Nakaseko, Y.、Yanagida, M.：“Cut1/分离酶 C 末端影响裂殖酵母尖核形成间期纺锤体极体的定位。”基因到细胞。

Fukumoto, Y., Hiyama, H., Yokoi, M., Nakaseko Y., Yanagida, M., Hanaoka, F.: "Two budding yeast RAD4 homologs in fission yeast play different. roles in the repair of UV-induced DNA damage."DNA repair. Vol.1 No.10. 833-845 (2002)

Fukumoto, Y.、Hiyama, H.、Yokoi, M.、Nakaseko Y.、Yanagida, M.、Hanaoka, F.：“裂殖酵母中的两种出芽酵母 RAD4 同源物在紫外线诱导的 DNA 修复中发挥不同的作用。

Nakamura, Tnagao, K, Nakaseko, Y, Yanagida M: "Ciut1/separase C-terminus affects spindle pole body positioning in interphase of fission yeast pointed nuclear formation"Genes to Cells. 7・11. 1113-1124 (2002)

Nakamura、Tnagao、K、Nakaseko、Y、Yanagida M：“Ciut1/分离酶 C 末端影响裂殖酵母尖核形成间期的纺锤体极体定位”《基因到细胞》1113-1124。