ANALYSIS OF CHROMOSOMESEGREGATION MECHANISM BY HUMAN CH-TOG RELATED GENES IN YEAST

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Fission yeast S. pombe dis1 mutants are defective in sister chromatids separation. Dis1 belongs to an evolutionarily conserved family of microtubule binding proteins, including human ch-TOG, frog XMAP215, fly minispindles and budding yeast Stu2p. We found Dis1-GFP signals are associated with cytoplasmic microtubules, spindle microtubules and spindle pole bodies. Surprisingly, as cells progress from metaphase to anaphase during mitosis, Dis1-GFP signals mimic those of kinetochore proteins such as Mis6, Mis12. Dis1 is the first fission yeast protein that associates with both microtubules and the centromeric DNA of the kinetochore. Dis1 may play a dual role by becoming a part of the kinetochores in an M-phase specific manner, possibly generating connections between kinetochores and microtubules.We have also shown that protein highly related to Dis1, called Mtc1/Alp14, behaves in a similar but not identical manner to Dis1. Their behavior suggests that these two related molecules have significant differences in interaction with microtubules.