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COMPREHEMSIVE ANALYSIS OF TUBULIN FUNCTIONAL GENE NETWORK IN YEAST

酵母微管蛋白功能基因网络的综合分析

基本信息

批准号：
16510145
负责人：
NAKASEKO Yukinobu
金额：
$ 2.05万
依托单位：
Kyoto University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2004
资助国家：
日本
起止时间：
2004 至 2006
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-16510145/
关键词：
mutants library microtubule comprehensive analysis cell cycle cell growth regulator suppressor analysis

项目摘要

Microtubule is one of the key machinery regulating cell cycle control, including cell growth, chromosome segregation. Although many genes and their cooperation are involved in regulation of tubulin, core component of microtubules, their functional networks are not known. Fission yeast Schizosaccharomyces pombe has played an important role for identification of cell cycle regulators and their functional analysis. Several approaches using mitotic mutant were extremely useful, and many genes were identified, and the mechanism of them became clear. We have constructed a randomly isolated mutant library of fission yeast and started systematic analysis about individual mutant genes and functional interaction among them. We have isolated 1015 strains having temperature sensitive mutation, not deletion mutation, for the purpose of analysis of most of essential genes. We have taken two kinds of different strategies to analyze tubulin regulatory network. In one approach, we investigated the genes interacting with dis1, a microtubule binding protein in fission yeast. In second approach we surveyed mutants of tubulin and their related genes. From these analysis we have found several new functional networks among them. These results are expected to elucidate the presence of multiple interactive gene networks that has not been analyzed in microtubule function.

微管是调节细胞周期控制（包括细胞生长、染色体分离）的关键机制之一。微管蛋白是微管的核心组成部分，其调控涉及多种基因及其相互作用，但其功能网络尚不清楚。裂殖酵母在细胞周期调控因子的鉴定和功能分析中发挥着重要作用。利用有丝分裂突变体的几种方法非常有用，许多基因被鉴定出来，它们的作用机制也逐渐明朗。我们构建了一个随机分离的裂殖酵母突变体库，并对单个突变基因及其功能相互作用进行了系统的分析。我们分离了1015株具有温度敏感突变而非缺失突变的菌株，以分析大部分必需基因。我们采用了两种不同的策略来分析微管蛋白的调控网络。在一种方法中，我们研究了与裂殖酵母中的微管结合蛋白dis1相互作用的基因。在第二种方法中，我们调查了微管蛋白及其相关基因的突变体。从这些分析中，我们发现了一些新的功能网络。这些结果有望阐明微管功能中尚未分析的多个相互作用的基因网络的存在。