Neurotrophic effects of Radicicol on Sensory Neurons

根底考对感觉神经元的神经营养作用

• 批准号: 13680848
• 负责人: SANO Mamoru
• 金额: $ 2.37万
• 依托单位: Kyoto Prefectural University of Medicine
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2001
• 资助国家: 日本
• 起止时间: 2001 至 2003
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-13680848/
• 关键词: radicicol; geldanamycin; Hsp90; NF108-15; N2a; DRG; ラディシコール; ゲルダナマイシン; ニューロトロフィン; 知覚神経節

Radicicol and geldanamycin, Hsp9O-binding antibiotics, potentiate neurite growth and survival of cultured sensory neurons from chick embryo. They did not exhibit trophic effects on the sensory neurons from embryonic and adult rats and mice. We show that the antibiotics induced the outgrowth of neurites in NG108-15 cells and N2A cells. Treatment of cells with these drugs decreased levels of Raf1,ERK1/2 and phosphorylated ERK1/2 time-dependently. Levels of Akt1 and CDK4 were slightly decreased. The neurite growth by the drugs was blocked by actynomycin D. The neurite growth of NG108-15 cells Geld might be due to a change in the synthesis of some specific protein(s).

与hsp90结合的抗生素Radicicol和geldanamycin对培养的鸡胚感觉神经元的神经突生长和存活有促进作用。它们对胚胎和成年大鼠和小鼠的感觉神经元没有表现出营养作用。结果表明，抗生素可诱导NG108-15细胞和N2A细胞的神经突生长。用这些药物治疗细胞降低了Raf1，ERK1/2和磷酸化ERK1/2的水平，这是时间依赖性的。Akt1和CDK4水平略有下降。NG108-15细胞的神经突生长可能与某些特定蛋白合成的改变有关。

项目成果

Sakuma K.: "Serum response factor plays an important role in the mechanically overloaded plantaris muscle of rats"Histochem.Cell Biol.. 119. 149-160 (2003)

Sakuma K.：“血清反应因子在大鼠机械超负荷的跖肌中发挥重要作用”Histochem.Cell Biol.. 119. 149-160 (2003)

Sakuma, K.: "Serum response factor plays an important role in the mechanically overloaded plantaris muscle of rats."Histochem. Cell Biol.. 119. 149-160 (2003)

Sakuma, K.：“血清反应因子在大鼠机械超负荷的跖肌中发挥着重要作用。”Histochem。

Sakuma K.: "Calcineurin is a potent regulator for skeletal muscle regeneration by association with NFATc1 and GATA-2"Acta Neuropathologica. 105. 271-280 (2003)

Sakuma K.：“钙调神经磷酸酶是骨骼肌再生的有效调节剂，与 NFATc1 和 GATA-2 相关”《神经病理学报》。

Sakuma K.: "Calcineurin is a potent regulator for skeletal muscle regeneration by association with NFATc1 and GATA-2"Acta Neurophathologica. 105. 271-280 (2003)

Sakuma K.：“钙调神经磷酸酶是骨骼肌再生的有效调节剂，与 NFATc1 和 GATA-2 相关”《神经病理学学报》。

Ishikawa, T.: "Peroxisomes exist in growth cones and move anterogradely and retrogradely in neurites of PC12D cells."Exp. Cell Res.. 266. 260-269 (2001)

Ishikawa, T.：“过氧化物酶体存在于生长锥中，并在 PC12D 细胞的神经突中顺行和逆行移动。”