Development of stimuli-sensitive polymer hydrogel as a scaffold for tissue engineering

开发刺激敏感聚合物水凝胶作为组织工程支架

• 批准号: 13680957
• 负责人: SUGIYAMA Kazuo
• 金额: $ 1.28万
• 依托单位: Kinki University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2001
• 资助国家: 日本
• 起止时间: 2001 至 2002
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-13680957/
• 关键词: poly(2-hydroxypropylmethacrylamide); poly(N-isopropylacrylamide); thermo-sensitive polymer; light-sensitive polymer; plasma irradiation-post polymerization; HeLa cells attachment and proliferation; thermo-stimuli cells detachment; light-stimuli cel

Tissue engineering is the recreation of a SD-tissue structure using a cell-seeded scaffold as a replacement for injured tissue to restore biological function. The development of an artifacial extracelluer matrix(ECM) enabling a regulation of cells attachment and proliferation by stimuli-response is becoming one of most important research field. The purpose of this project was 1.preparation and characterization of novel stimuli-response (heat, light, and pH) polymer / hydrogel, 2.development of immobilizing technique of these polymers onto commodity polymers, and examination of cells proliferation on the polymer modified matrix and detachment by some stimulus. The following results were obtained; 1-(1)Thermo- or light- sensitive polymers were prepared from biocompatible poly(2-hydroxypropylmethacrylamide)[poly(HPMA)] with azobenzene moieties or chlorophyllin-Cu complex. 1-(2)Thermo- and pH senstive polymer(hydrogel) were also obtained from N-isopropylacrylamide(NiPAAm) and amphoteric o- … More methacryloyl-L-amino acid. 1-(3)The release of dansyl-s-phenyl alanine as a drug model was regulated by thermo-stimuli from thermo-sensitive poly(HPMA- co-N-methacryloyl-s-phenylalanie methyl ester) gel. 1-(4)The biocompatibility of poly[2-(methacryloyloxy)ethyl phosphorylchline] as an ECM model was dependent on the structure of polymer chain in water and/or molecular weight. 2-(1)The utilization of novel polymethacrylate or polystyrene containing acrylamide derivatives as an adhesive primers was effective for adhesive bonding between non-polar polyolefine and polar material. 2-(2)The thermo- or light-sensitive polymer was grafted onto polypropylene (PP) or polyethyleneterephtharate(PET) film by using Ar plasma irradiation and post polymerization technique. 2-(3)The attachment and detachment of polystyrene microspheres as a cell model was able to regulate photochemically and thermally on the PP film, 2-(4)The proliferation of HeLa cells on the thermo-sensitive poly(HPMA) grafted PET film(g-PET) was almost as much as that of a polystyrene cell culture dish. 2-(5)The degree of thermal detachment of HeLa cells on the g-PET with a decrease in culturing temperature from 37 ℃ to 4 ℃ after the proliferation was much higher than that of thermo-sensitive poly(NiPAAm) grafted PET. Less

组织工程是使用细胞接种的支架作为受损组织的替代物来重建SD组织结构以恢复生物功能。人工细胞外基质（ECM）的开发是通过刺激反应来调节细胞粘附和增殖的重要研究领域之一。该项目的目的是1.新型刺激响应（热、光和pH）聚合物/水凝胶的制备和表征，2.开发这些聚合物在商品聚合物上的固定技术，并检查细胞在聚合物修饰的基质上的增殖和脱落。一些刺激。（1）由具有生物相容性的聚（2-羟丙基甲基丙烯酰胺）[聚（HPMA）]与偶氮苯或叶绿素铜配合物制备了热敏或光敏聚合物。1-（2）以N-异丙基丙烯酰胺（NiPAAm）和两性邻苯二甲酸二异丙酯（NPAAm）为原料，合成了具有温敏性和pH敏感性的聚合物（水凝胶）。 ...更多信息 甲基丙烯酰-L-氨基酸。1-（3）以丹磺酰-s-苯丙氨酸为药物模型，用温敏性聚（HPMA-co-N-甲基丙烯酰-s-苯丙氨酸甲酯）凝胶，通过热刺激调控丹磺酰-s-苯丙氨酸的释放。聚[2-（甲基丙烯酰氧基）乙基磷酰胆碱]作为ECM模型的生物相容性取决于聚合物链在水中的结构和/或分子量。2-（1）使用含有丙烯酰胺衍生物的新型聚甲基丙烯酸酯或聚苯乙烯作为粘合剂底漆对于非极性聚烯烃和极性材料之间的粘合剂粘结是有效的。2-（2）采用Ar等离子体辐照和后聚合技术，将温敏或光敏聚合物接枝到聚丙烯（PP）或聚对苯二甲酸乙二酯（PET）薄膜上。2-（3）作为细胞模型的聚苯乙烯微球在PP膜上的贴附和脱离具有光化学和热调节作用。2-（4）HeLa细胞在温敏性聚（HPMA）接枝PET膜（g-PET）上的增殖几乎与聚苯乙烯细胞培养皿一样多。2-（5）HeLa细胞在g-PET上增殖后，随着培养温度从37 ℃降至4 ℃，其热脱附程度远高于温敏性聚（NiPAAm）接枝PET。少

项目成果

白石浩平, 山路 毅, 杉山一男: "N-2-ヒドロキシプロピルメタクリルアミドとMMAからなる両親媒性ポリマーの調製と温度応答性"高分子学会予稿集. 50巻・14号. 3769 (2001)

Kohei Shiraishi、Takeshi Yamaji、Kazuo Sugiyama：“由 N-2-羟丙基甲基丙烯酰胺和 MMA 组成的两亲性聚合物的制备和温度响应性”聚合物科学技术学会论文集，第 50 卷，第 14 期。3769（2001 年）。

Kazuo Sugiyama: "Characterization and application of polypropylene film modified with stimuli-sensitive copolymers using Ar plasma-post polymerization technique"Journal of Applied Polymer Science. (in press). (2003)

Kazuo Sugiyama：“使用Ar等离子体后聚合技术对刺激敏感共聚物改性聚丙烯薄膜的表征和应用”应用聚合物科学杂志。

Kohei Shiraishi: "Application of poly(alkoxymethyl acrylamide) to primers in adhesive bonding of polypropylene"Journal of the Society of Material Science Japan. Vol.52. 319-324 (2003)

Kohei Shiraishi：“聚烷氧基甲基丙烯酰胺在聚丙烯粘合底漆中的应用”日本材料学会杂志。

白石浩平: "ポリ(アルコシキメチルアクリルアミド)の接着表面処理剤への応用"材料. (in press). (2003)

Kohei Shiraishi：“聚烷氧基甲基丙烯酰胺在粘合剂表面处理剂中的应用”材料（2003 年出版）。

杉山一男: "プラズマ照射・ポスト重合法で感温性ポリマーをグラフトしたPETフィルムへのHeLa細胞の接着・増殖と温度刺激剥離"高分子学会予稿集. 51巻・14号. 3712 (2002)

Kazuo Sugiyama：“通过等离子体辐射/后聚合方法将 HeLa 细胞粘附/增殖和温度刺激分离到接枝有热敏聚合物的 PET 膜上”，聚合物科学技术学会论文集，第 51 期，第 14 期。3712。 (2002)