Development of stimuli-sensitive polymer hydrogel as a scaffold for tissue engineering

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Tissue engineering is the recreation of a SD-tissue structure using a cell-seeded scaffold as a replacement for injured tissue to restore biological function. The development of an artifacial extracelluer matrix(ECM) enabling a regulation of cells attachment and proliferation by stimuli-response is becoming one of most important research field. The purpose of this project was 1.preparation and characterization of novel stimuli-response (heat, light, and pH) polymer / hydrogel, 2.development of immobilizing technique of these polymers onto commodity polymers, and examination of cells proliferation on the polymer modified matrix and detachment by some stimulus. The following results were obtained; 1-(1)Thermo- or light- sensitive polymers were prepared from biocompatible poly(2-hydroxypropylmethacrylamide)[poly(HPMA)] with azobenzene moieties or chlorophyllin-Cu complex. 1-(2)Thermo- and pH senstive polymer(hydrogel) were also obtained from N-isopropylacrylamide(NiPAAm) and amphoteric o- … More methacryloyl-L-amino acid. 1-(3)The release of dansyl-s-phenyl alanine as a drug model was regulated by thermo-stimuli from thermo-sensitive poly(HPMA- co-N-methacryloyl-s-phenylalanie methyl ester) gel. 1-(4)The biocompatibility of poly[2-(methacryloyloxy)ethyl phosphorylchline] as an ECM model was dependent on the structure of polymer chain in water and/or molecular weight. 2-(1)The utilization of novel polymethacrylate or polystyrene containing acrylamide derivatives as an adhesive primers was effective for adhesive bonding between non-polar polyolefine and polar material. 2-(2)The thermo- or light-sensitive polymer was grafted onto polypropylene (PP) or polyethyleneterephtharate(PET) film by using Ar plasma irradiation and post polymerization technique. 2-(3)The attachment and detachment of polystyrene microspheres as a cell model was able to regulate photochemically and thermally on the PP film, 2-(4)The proliferation of HeLa cells on the thermo-sensitive poly(HPMA) grafted PET film(g-PET) was almost as much as that of a polystyrene cell culture dish. 2-(5)The degree of thermal detachment of HeLa cells on the g-PET with a decrease in culturing temperature from 37 ℃ to 4 ℃ after the proliferation was much higher than that of thermo-sensitive poly(NiPAAm) grafted PET. Less