Molecular neurobiology of the GABAB receptor: Studies of heteromeric receptor function and signalling

GABAB 受体的分子神经生物学：异聚受体功能和信号传导的研究

• 批准号: DP0344875
• 负责人: Prof Philip Beart
• 金额: $ 16.75万
• 依托单位: The University of Melbourne
• 依托单位国家: 澳大利亚
• 项目类别: Discovery Projects
• 财政年份: 2003
• 资助国家: 澳大利亚
• 起止时间: 2003-08-15 至 2006-12-31
• 项目状态: 已结题
• 来源: https://dataportal.arc.gov.au/RGS/Web/Grants/DP0344875
• 关键词: Molecular; neurobiology; GABAB; receptor; Studies

The G protein-coupled receptor (GPCR) for the inhibitory transmitter gamma- aminobutyric acid (GABA) is a unique heterodimer. Molecular analyses will be undertaken to provide insights into its signalling mechanisms and functional regulation. Investigations employing point mutant and chimeric receptors will analyse how ligand binding to the extracellular domain of the GABA-BR1 subunit triggers G protein-coupling to the intracellular portion of the GABA-BR2 subunit. Focus will be on different modes of GPCR signalling, including constitutive activity and roles for membrane and cytosolic regulatory proteins. Targeted studies of GABAB receptor subunits will provide new information on the mechanistic regulation of GPCR signalling.

抑制性递质γ-氨基丁酸（GABA）的G蛋白偶联受体（GPCR）是一种独特的异源二聚体。将进行分子分析，以深入了解其信号传导机制和功能调节。 调查采用点突变和嵌合受体将分析如何配体结合GABA-BR 1亚基的胞外结构域触发G蛋白偶联GABA-BR 2亚基的胞内部分。重点将是不同模式的GPCR信号，包括膜和胞质调节蛋白的组成活动和作用。对GABAB受体亚单位的靶向研究将为GPCR信号传导的机制调控提供新的信息。