Structure and dynamics of a multiprotein-mRNA complex involved in the regulation of gene expression

参与基因表达调控的多蛋白-mRNA复合物的结构和动力学

• 批准号: DP0345376
• 负责人: Prof Matthew Wilce
• 金额: $ 23.94万
• 依托单位: Monash University
• 依托单位国家: 澳大利亚
• 项目类别: Discovery Projects
• 财政年份: 2003
• 资助国家: 澳大利亚
• 起止时间: 2003-03-18 至 2006-12-31
• 项目状态: 已结题
• 来源: https://dataportal.arc.gov.au/RGS/Web/Grants/DP0345376
• 关键词: Structure; dynamics; multiprotein; mRNA; complex

RNA/protein interactions are now recognised as a major control point in the regulation of gene-expression. Proteins such as HuR and the poly(C)-binding proteins (PCBPs) act to stabilise and transport specific messenger (m)RNAs, and thus determine their translation levels. In contrast to such an important function, very little is known about these protein/mRNA interactions at an atomic level. The current study will investigate the structural and biophysical properties of a recently discovered HuR/PCBP/mRNA complex implicated in the regulation of androgen receptor expression. This information has the potential to assist in the development of drugs to reduce AR expression in prostate cancer.

RNA/蛋白质相互作用现在被认为是基因表达调控的主要控制点。 HuR 和聚 (C) 结合蛋白 (PCBP) 等蛋白质可稳定和运输特定信使 (m)RNA，从而确定其翻译水平。与如此重要的功能相比，人们对这些蛋白质/mRNA在原子水平上的相互作用知之甚少。目前的研究将调查最近发现的与雄激素受体表达调节有关的 HuR/PCBP/mRNA 复合物的结构和生物物理特性。该信息有可能有助于开发减少前列腺癌中 AR 表达的药物。