Protein Over-Expression/Purification and Macromolecular Structure Determination by X-Ray Diffraction

通过 X 射线衍射进行蛋白质过表达/纯化和大分子结构测定

• 批准号: LE0346892
• 负责人: Prof Matthew Wilce
• 金额: $ 47.03万
• 依托单位: The University of Western Australia
• 依托单位国家: 澳大利亚
• 项目类别: Linkage Infrastructure, Equipment and Facilities
• 财政年份: 2003
• 资助国家: 澳大利亚
• 起止时间: 2003-03-26 至 2005-06-14
• 项目状态: 已结题
• 来源: https://dataportal.arc.gov.au/RGS/Web/Grants/LE0346892
• 关键词: Protein; Over; Expression; Purification; Macromolecular

This proposal seeks funds for state-of-the-art facilities for protein over-expression and macromolecular X-ray diffraction. This will build upon recent initiatives within the collaborating institutions in the field of Structural Biology. It will enable research groups in Perth to pursue the large-scale production of important proteins and to conduct high-resolution structural studies using X-ray crystallographic techniques. This technology, which is one of the most important tools in modern biology, provides unique insights into the chemical mechanisms of biological macromolecules and will significantly enhance a great breadth of biological research in Western Australia.

这项提案寻求为最先进的蛋白质过度表达和大分子X射线衍射设施提供资金。这将建立在结构生物学领域合作机构最近的倡议基础上。它将使珀斯的研究小组能够追求重要蛋白质的大规模生产，并使用X射线结晶学技术进行高分辨率结构研究。这项技术是现代生物学中最重要的工具之一，它为生物大分子的化学机制提供了独特的见解，并将显著提高西澳大利亚生物学研究的广度。