Analyses on the pollination system of tree species with various regeneration characteristics

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In this research project we analyzed pollination processes of tree species with various regeneration characteristics with the aid of microsatellite markers that had been developed for tree species with various pollination system and seed dispersal characteristics such as Aesculus turbinata (insect pollination and seed dispersal by the gravity and mammals), Magnolia obovata (insect pollination and avian seed dispersal), Rhododendron metternichii var.hondoense (insect pollination and seed dispersal by wind), Quercus myrsinifolia (wind pollination and seed dispersal by the gravity and mammals), Dipterocarpus tempehens (insect pollination and seed dispersal by wind). In addition to these markers, we newly developed for Kalopanax pictus (wind pollination and avian seed dispersal) and Cercidiphyllum japonicum (wind pollination and seed dispersal by wind) in the present study and evaluated the polymorphism in long term large scale research sites. Long term and large scale research sites were maintained and genotyping was made for every reproductive individuals. For the research of Magnolia obovata, Kalopanax pictus, Aesculus turbinata, the area of research site was extended as large as several hundred ha, and facility to reach the crown of tree was developed. Analyses with microsatellite markers revealed many interesting characteristics of tree pollination, such as idiosyncratic pollination to individual trees and flowers of Magnolia obovata, effect of genetic structure to pollen dispersal distances of Aesculus turbinate, and very long distance pollen movement found in Dipterocarpus tempehens in the tropical rain forests.