Development of Micro pH Sensor for Diagnosis of Dental Caries Activity

用于诊断龋齿活动的微型pH传感器的开发

• 批准号: 14207079
• 负责人: TAGAMI Junji
• 金额: $ 26.79万
• 依托单位: Tokyo Medical and Dental University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (A)
• 财政年份: 2002
• 资助国家: 日本
• 起止时间: 2002 至 2004
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-14207079/
• 关键词: active carious dentin; arrested carious dentin; pH; micro-pH sensor; ISFET; pH-imaging microscope; caries activity; Minimal Intervention; う蝕活動性; pHイメージング光走査型化学顕微鏡; 小児う蝕象牙質; PCR法; 微小引張り強さ; MEMS技術; ISFETセンサー; X線透過性; EPMA; 陽極接合

The pH values of extracted carious and intact dentin have been investigated visually and quantitatively using a pH-imaging microscope (Kitasako et al., 2002). The pH distribution of carious dentin was shown to be lower than that of intact dentin. In addition, there was a significant correlation between a decrease in pH and mineral loss in active dentinal caries lesions (Hiraishi et al.2003). Nakajima et al. (2004) also reported the distributions of the minerals, Ca, P, and Mg, at the adhesive interface to caries-affected dentin were different from normal dentin. However, the pH-imaging sensor is too large for evaluating the pH of caries in the oral cavity directly. A smaller version would allow in vivo pH-imaging characterization of caries lesions in the oral cavity. Recently, a micro-pH sensor using an Ion Sensitive Field Effect Transistor (ISFET) has been developed to overcome the problem of the glass and antimony electrodes (Murakami et al., 2005). Twenty extracted carious teeth were divided into two groups, active or arrested caries. The surface pH values of carious dentin were measured using three methods : surface pH directly measured using the micro-pH sensor ; sectioned teeth using a pH-Imaging microscope or the micro-pH sensor. For all techniques, the lowest pH values of the active and arrested carious dentin (5.3-6.5) were less than those of intact dentin (6.8-7.4). Comparison of pH data from pH-Imaging microscope or micro-pH sensor, which measured the same pH point, a high agreement (p=0.890) was confirmed. For all techniques, there was a statistically significant differences of pH values between active and arrested dentinal caries. Because this micro-pH sensor can evaluate caries activity on each tooth, it might have the potential to aid in assessment of caries lesions and then the subsequent type of clinical management using the concepts of Minimal Intervention.

已经使用pH成像显微镜视觉和定量地研究了提取的龋坏和完整牙本质的pH值（Kitasako等人，2002年）。龋坏牙本质的pH值分布低于完整牙本质。此外，在活动性牙本质龋损中，pH值降低与矿物质损失之间存在显著相关性（Hiraishi等，2003）。Nakajima等（2004）还报道了在龋损牙本质的粘附界面处，矿物质Ca、P和Mg的分布与正常牙本质不同。然而，pH成像传感器对于直接评估口腔中龋齿的pH而言太大。一个较小的版本将允许在体内pH成像表征口腔中的龋齿病变。最近，已经开发了使用离子敏感场效应晶体管（ISFET）的微pH传感器以克服玻璃和锑电极的问题（Murakami等人，2005年）。将20颗拔除的龋牙分为两组，活动龋组和停止龋组。龋坏牙本质的表面pH值的测量使用三种方法：表面pH值直接测量使用微型pH传感器;切片牙齿使用pH成像显微镜或微型pH传感器。对于所有的技术，最低pH值的活动和停止龋牙本质（5.3-6.5）小于完整的牙本质（6.8-7.4）。比较来自pH成像显微镜或微型pH传感器的pH数据，其测量相同的pH点，证实了高度一致性（p=0.890）。对于所有的技术，有一个统计学上的显着差异的pH值之间的活动性和停止牙本质龋。由于这种微型pH传感器可以评估每颗牙齿上的龋齿活动，因此它可能有助于评估龋齿病变，然后使用最小干预概念进行后续类型的临床管理。

项目成果

北迫勇一, 田上順次: "pHイメージング顕微鏡による新しいう蝕診断法の開発"口腔病学会誌. 69・4. 54 (2002)

Yuichi Kitasako、Junji Tagami：“利用 pH 成像显微镜开发新的龋齿诊断方法”口腔医学会杂志 69・4.54（2002 年）。

北迫勇一, 田上順次, 平石典子, 奥田真実子, 二階堂徹: "むし歯研究におけるイメージング分析の活用方法"Readout. 24. 37-41 (2002)

Yuichi Kitasako、Junji Tagami、Noriko Hiraishi、Mariko Okuda、Toru Nikaido：“如何在龋齿研究中利用成像分析”Readout。

小型pHセンサーによるヒト抜去う蝕象牙質のう蝕活動性診断

使用小型 pH 传感器诊断人类龋齿牙本质的龋齿活动

• 发表时间: 2005
• 期刊: 接着歯学 Vol.23
• 作者: 村上景子;北迫勇一;山貫幹人;野村聡;中西剛;田上順次
• 通讯作者: 田上順次

Surface analysis of dentinal caries in primary teeth using a pH-imaging microscope

• DOI: 10.4012/dmj.23.628
• 发表时间: 2004-12-01
• 期刊: DENTAL MATERIALS JOURNAL
• 影响因子: 2.5
• 作者: Nikaido, T;Moriya, K;Tagami, AJ
• 通讯作者: Tagami, AJ

Elemental distributions and microtensile bond strength of adhesive interface to normal and caries-affected dentin.

正常牙本质和受龋齿影响的牙本质粘合界面的元素分布和微拉伸粘合强度。

• 发表时间: 2005
• 期刊: Journal of Biomedical Materials Research Part B 72(2)
• 作者: Nakajima M;Kitasako Y;Okuda M;Foxton RM;Tagami J.
• 通讯作者: Tagami J.