Caries activity assessment using the micro pH sensor and Real time PCR for cariogenic organisms obtained from the lowest pH area of caries dentin

使用微型 pH 传感器和实时 PCR 评估从龋齿牙本质最低 pH 区域获得的致龋微生物的龋齿活性

• 批准号: 17390507
• 负责人: TAGAMI Junji
• 金额: $ 10.39万
• 依托单位: Tokyo Medical and Dental University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2005
• 资助国家: 日本
• 起止时间: 2005 至 2007
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-17390507/
• 关键词: caries; pH censer; demineralization; streptococci; dentin; PCR; white spot lesion; remineralization; buffer capacity; saliva

The aim of this clinical study, was to measure the surface pH of carious dentin using the micro pH sensor directly. 25 samples obtained from 20 patients (mean age: 53.8) were classified into active or arrested carious dentin before the pH assessments. If sample was not classified both type of carious dentin, it was recognized pre-active carious dentin. The surface pH of intact enamel was also measured using this micro pH sensor. Moreover, saliva, plaque and carious dentin samples were obtained from same subjects to evaluate carious organism's populations using Real time PCR methods. The surface pH was range from pH 3.8 to pH 5.2 for active carious dentin, range from pH 5.8 to pH 6.3 for arrested carious dentin, and pH 5.4 for pre-active carious dentin respectively. There were statistical differences on the surface pH value between active and arrested carious dentin, and pre-active and arrested carious dentin (p<0.05). On the other hand, the surface pH values after caries removing was about pH 6.4 for all types of carious dentin. Because the critical pH of the crystal of carious affected dentin is pH 6.4, that pH value after carious removing might show the inner layer of carious dentin. From the results of pH analysis, this micro pH sensor could be useful for clinical assessments for the carious activity. Although the genomic DNA of S. mutans was detected all carious samples, there was not statistical differences on the rate of genomic DNA for the S. mutans between active and arrested carious dentin. There were non detected samples for the genomic DNA of S. sobrinus among active carious dentin. The rate of genomic DNA of S. sobrinus related to the active carious dentin was lower than that of arrested carious dentin.

本临床研究的目的是利用微型pH传感器直接测量龋坏牙本质表面的pH值。从20名患者（平均年龄：53.8）中获得的25个样本在pH评估之前被分类为活动或停止龋坏牙本质。如果样本不能被分类为两种类型的龋坏牙本质，则被认为是前活动龋坏牙本质。使用该微型pH传感器还测量了完整釉质的表面pH。另外，采用真实的时间PCR方法，对同一受试者的唾液、牙菌斑和龋坏牙本质样本进行龋坏菌群的检测。活动龋牙本质表面pH值范围为3.8 ~ 5.2，停龋牙本质表面pH值范围为5.8 ~ 6.3，活动前龋牙本质表面pH值范围为5.4。活动期与停龋期牙本质表面pH值与活动期与停龋期牙本质表面pH值比较差异有统计学意义（p<0.05）。另一方面，对于所有类型的龋性牙本质，去除龋后的表面pH值约为pH 6.4。由于龋蚀牙本质晶体的临界pH值为pH6.4，因此，去龋后的pH值可显示龋蚀牙本质的内层。从pH值分析结果来看，该微型pH传感器可用于临床评价龋活性。虽然S.所有龋损标本均检出变形链球菌，各菌株基因组DNA检出率差异无统计学意义。在活跃的和静止的龋坏牙本质之间的变异体。未检出S.活动性龋坏牙本质间的远缘。S.与活动龋牙本质相关的sobrinus明显低于静止龋牙本质。

项目成果

A technique using resin composite with orthodontic wire to replacea missing tooth rapidly

树脂复合材料与正畸钢丝快速替代缺失牙齿的技术

• 发表时间: 2008
• 期刊: Dental Traumatology 24
• 作者: Kitasako Y;Ikeda M;Burrow MF and Tagami J
• 通讯作者: Burrow MF and Tagami J

Quantitative assessment for stimulated saliva flow rate and buffering capacity in relation to different ages

• DOI: 10.1016/j.jdent.2006.01.004
• 发表时间: 2006-10-01
• 期刊: JOURNAL OF DENTISTRY
• 影响因子: 4.4
• 作者: Moritsuka, Michiyo;Kitasako, Yuichi;Nomura, Satoshi
• 通讯作者: Nomura, Satoshi

Enamel bonding of self-etch and phosphoric acid-etch orthodontic adhesive systems.

自蚀刻和磷酸蚀刻正畸粘合剂系统的牙釉质粘合。

• 发表时间: 2007
• 期刊: Dental Material Journal 26
• 作者: Kitayama S;Nikaido T;Ikeda M;Foxton RM;Tagami J
• 通讯作者: Tagami J

Migration and particle from hard-setting Ca(OH)_2 and self-etching adhesive resin following direct pulp capping

直接盖髓后硬固性 Ca(OH)_2 和自酸蚀粘合树脂的迁移和颗粒

• 发表时间: 2006
• 期刊: American Journal of Dentistry 19(6)
• 作者: Kitasako Y;Shibata S;Tagami J
• 通讯作者: Tagami J

Caries activity assessment using the micro pH sensor and Real time PCR for cariogenic organisms obtained from the 1 owest pH area of caries dentin

使用微型 pH 传感器和实时 PCR 对从龋齿牙本质 1 个最低 pH 区域获得的致龋微生物进行龋齿活动评估

• 发表时间: 2007
• 作者: Kitasako Y;Shida K;Nikaido T;Matin K;Tagami J.
• 通讯作者: Tagami J.