Gene-engineered hybrid prostheses for small vascular reconstruction

用于小血管重建的基因工程混合假体

• 批准号: 14380387
• 负责人: HIRATA Satoshi
• 金额: $ 4.86万
• 依托单位: Asahikawa Medical College
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2002
• 资助国家: 日本
• 起止时间: 2002 至 2005
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-14380387/
• 关键词: small caliber proshteses; hybrid artificial graft; gene transfer; alloeraft; decellularized graft; HVJ; HCG; intimal hyperplasia; 血管新生; 内皮細胞; 肝細胞増殖因子HGF; HGF(肝細胞増殖因子); 人工血管; 小口径; hepatocyte growth factor; 平滑筋細胞

Difficulty of the healing process between human being and artificial materials is main cause of graft failure of artificial arterial prostheses in small caliber arterial reconstruction. This research project has been perform in order to overcome this problem and establish small caliber prostheses.To resolve this difficult situation, we perform the following research and try to 1)establish of gene transfer methods for vascular cells, 2)developement of small caliber arterial graft.1)Establish of gene transfer methods for vascular wall cells : We perform gene transfer to blood vessel by three different methods including pressure method, HVJ-Envelope vector method, and ultrasonic method, and evaluate the transfer efficiency and degree of structural damage of vessel wall. As the results of this research, the HVJ-Envelope method is most effective and less invasive for gene transfer to vessel wall cells.2)Development of small caliber prostheses : smooth muscle cell seeding hybrid artificial p … More rostheses was evaluated in vitro as well as in vivo. Human cell growth as well as cell repopulation on the artificial material is much inferior compared to other species, even if growth factors supply or hepatic growth factor gene transfer. Anti-thrombotic characteristics is also unsolved problem for small caliber artificial prostheses. Therefore, hybrid artificial grafts were all occluded in canine femoral arterial reconstruction model. On the other hands, biologic materials such as arterial allograft have superior characteristics regarding antithrombogenecity and cell repopulation.In conclusion, it is still difficult to establish small caliber artificial prostheses because of human system against artificial materials. However, the biological materials such as allograft have promising characteristics in terms of anti-thrombogenicity and cell repopulation. So, tissue engineered allograft such as decellularized allograft or gene transferred allograft are next target of small caliber prostheses. Less

人类和人造物质之间的康复过程的困难是小口径人工伪像的人工伪像失败的主要原因。 This research project has been perform in order to overcome this problem and establish small caliber prostheses.To resolve this difficult situation, we perform the following research and try to 1)establish of gene transfer methods for vascular cells, 2)development of small caliber artefact.1)Establish of gene Transfer methods for vascular wall cells: We perform gene transfer to blood vessel by three different methods including pressure method, HVJ-Envelope vector method, and ultrasonic method, and evaluate容器壁的转移效率和结构损伤程度。作为这项研究的结果，HVJ-Envelope方法对基因转移到血管壁细胞的转移最有效，侵入性较小。2）小口径假体的开发：平滑肌细胞播种杂种杂交人造P…在体外和体内评估更多的玫瑰花塞。与其他物种相比，人造物质的人类细胞生长以及细胞重生远远低，即使生长因子供应或肝炎生长因子基因转移。对于小口径人造假体而言，抗栓性特征也是未解决的问题。因此，杂种人造移植物均在犬股动脉重建模型中遮住。另一方面，诸如人工制品之类的生物学材料在抗积体和细胞重生方面具有较高的特征。总而言，由于人类对人造物质的人类系统，由于人类的系统，建立小口径人工假体仍然很难。然而，在抗超凡性和细胞再生方面，诸如合金等生物学材料具有有希望的特征。因此，组织工程合金（例如脱细胞合金或基因的同种异体移植物）是小口径假体的下一个目标。较少的