PHOTOSYNTHESIS AT DAWN

黎明时的光合作用

• 批准号: 14390051
• 负责人: INOUE Kazuhito
• 金额: $ 8万
• 依托单位: Kanagawa University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2002
• 资助国家: 日本
• 起止时间: 2002 至 2005
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-14390051/
• 关键词: PHOTOSYNTHESIS; PHOTOSYNTHETIC BACTERIA; PIGMENT; EVOLUTION; CHLOROPHYLL; CYANOBACTERIA; ENERGY METABOLISM; ENVIROMENTAL ADAPTATION; 光合成細; カロテノイド

The origin and evolution of photosynthesis have long remained enigmatic. To probe early evolutionary history of photosynthesis, we have investigated (B)Chl synthesis, biogenesis of photosynthetic apparatus, and electron transport of purple bacteria, green sulfur bacteria, heliobacteria and cyanobacteria. The summary of this study are as follows (Only the main results are shown).(1)Chlorophyll synthesis : A number of genes involved in (B)Chl synthesis were isolated from various phototrophic prokaryote. Homologues of bchF and bchG from green-sulfur bacterium Chlorobium tepidum were introduced and expressed in non-sulfur bacterium Rhodobacter capsulatus and cyanobacteria Synechocystis sp.PCC6803. Novel pigments accumulated in the cells of R.capsulatus and Synechocystis.(2)Biogenesis of photosynthetic apparatus : Assembly factors of Iron-sulfur cluster, IscR and IscS, from C.tepidum were overexpressed and characterized. Ferredoxin and iron-sulfur type RC were isolated from heliobacteria and they supported NADP^+ photoreduction in vitro. Nitrogenase-like enzyme, Dark POR, was isolated from R.capsulatus and characterized its biochemical properties.(3)Electron transport : Bound cytochrome of Rubrivivax gelatinosus belongs tri-heme type, while that of Blastochloris viridis is tetra-heme type. Chimeric RC of R.gelatinosus which contained B.viridis cytochrome subunit was constructed and investigated their electron transport.

光合作用的起源和进化长期以来一直是个谜。为了探究光合作用的早期进化史，我们研究了 (B)Chl 合成、光合器官的生物发生以及紫色细菌、绿硫细菌、日光细菌和蓝细菌的电子传递。本研究的概要如下(仅显示主要结果)。(1)叶绿素合成:从多种光养原核生物中分离出一些参与(B)Chl合成的基因。引入绿硫细菌温热绿菌的 bchF 和 bchG 同源物，并在非硫细菌荚膜红杆菌和蓝细菌集胞藻 PCC6803 中表达。新色素在R.capsulatus和Synechocystis细胞中积累。(2)光合器官的生物发生：来自C.tepidum的铁硫簇组装因子IscR和IscS被过表达和表征。铁氧还蛋白和铁硫型 RC 是从日光细菌中分离出来的，它们在体外支持 NADP^+ 光还原。从R.capsulatus中分离得到类固氮酶Dark POR，并对其生化性质进行了表征。(3)电子转运：Rubrivivax gelatinosus的结合细胞色素属于三血红素型，而Blastochoris viridis的结合细胞色素属于四血红素型。构建了含有 B.viridis 细胞色素亚基的 R.gelatinosus 嵌合 RC，并研究了其电子传递。

项目成果

Overexpression of the ferredoxin gene located on the downstream of nif operon in the green sulfur bacterium Chlorobium tepidum

绿色硫细菌温绿球菌中nif操纵子下游铁氧还蛋白基因的过表达

• 发表时间: 2005
• 期刊: Photosynthesis : Fundamental Aspects to Global Perspectives (ed by A.van der Est, D.Bruce)
• 作者: Furusawa;T.;Seo;D.;Sakurai;H.;Inoue;K.
• 通讯作者: K.

Hirabayashi H., Ishi T., Takaichi S., Inoue K., Uehara: "The role of carotenoids in the photoadaptation of the brown-colored sulfur bacterium Chlorobium phaeobacteroides"Photochemistry and photobiology. 79(3)(掲載決定). (2004)

Hirabayashi H.、Ishi T.、Takaichi S.、Inoue K.、Uehara：“类胡萝卜素在棕色硫细菌 Chlorobium phaeobacteroides 的光适应中的作用”光化学和光生物学 79(3)（出版决定）。 ）

Minor but key chloirophylls in photosystem II.

光系统 II 中次要但关键的叶绿素。

• 发表时间: 2005
• 期刊: Photosyn.Res. 84
• 作者: Kobayashi M.;Watanabe S.Gotoh;Koizumi H.;Itoh Y.;Akiyama M.
• 通讯作者: Akiyama M.

Electron interaction between redox cofactors in photosynthetic reaction centers.

光合作用反应中心氧化还原辅助因子之间的电子相互作用。

• 发表时间: 2004
• 期刊: J.Biol.Chem. 279
• 作者: Alric J.;Cuni A;Maki H.;Nagashima K.V.P;Vermeglio A.;Rappaport F.
• 通讯作者: Rappaport F.

Identification of the primary electron donor in PSII of the Chl d-dominated cyanobacterium Acaryochloris mariana.

叶绿素 d 主导的蓝藻 Acaryocholis mariana 的 PSII 中初级电子供体的鉴定。

• 发表时间: 2004
• 期刊: FEBS Lett. 556
• 作者: Mimuro M.;Akimoto S.;Gotoh T.;Yokono M.;Akiyama M.;Tsuchiyia T.;Miyashita H.;Kobayashi M.;Yamazaki I.
• 通讯作者: Yamazaki I.