PHOTOSYNTHESIS AT DAWN

黎明时的光合作用

• 批准号: 14390051
• 负责人: INOUE Kazuhito
• 金额: $ 8万
• 依托单位: Kanagawa University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2002
• 资助国家: 日本
• 起止时间: 2002 至 2005
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-14390051/
• 关键词: PHOTOSYNTHESIS; PHOTOSYNTHETIC BACTERIA; PIGMENT; EVOLUTION; CHLOROPHYLL; CYANOBACTERIA; ENERGY METABOLISM; ENVIROMENTAL ADAPTATION; 光合成細; カロテノイド

The origin and evolution of photosynthesis have long remained enigmatic. To probe early evolutionary history of photosynthesis, we have investigated (B)Chl synthesis, biogenesis of photosynthetic apparatus, and electron transport of purple bacteria, green sulfur bacteria, heliobacteria and cyanobacteria. The summary of this study are as follows (Only the main results are shown).(1)Chlorophyll synthesis : A number of genes involved in (B)Chl synthesis were isolated from various phototrophic prokaryote. Homologues of bchF and bchG from green-sulfur bacterium Chlorobium tepidum were introduced and expressed in non-sulfur bacterium Rhodobacter capsulatus and cyanobacteria Synechocystis sp.PCC6803. Novel pigments accumulated in the cells of R.capsulatus and Synechocystis.(2)Biogenesis of photosynthetic apparatus : Assembly factors of Iron-sulfur cluster, IscR and IscS, from C.tepidum were overexpressed and characterized. Ferredoxin and iron-sulfur type RC were isolated from heliobacteria and they supported NADP^+ photoreduction in vitro. Nitrogenase-like enzyme, Dark POR, was isolated from R.capsulatus and characterized its biochemical properties.(3)Electron transport : Bound cytochrome of Rubrivivax gelatinosus belongs tri-heme type, while that of Blastochloris viridis is tetra-heme type. Chimeric RC of R.gelatinosus which contained B.viridis cytochrome subunit was constructed and investigated their electron transport.

光合作用的起源和进化一直是个谜。为了探索光合作用的早期进化历史，我们研究了紫色细菌、绿色硫细菌、日光细菌和蓝藻的（B）叶绿素合成、光合机构的生物发生和电子传递。本研究总结如下（仅显示主要结果）。（1）叶绿素合成：从各种光合原核生物中分离到了一系列参与（B）Chl合成的基因。将绿色硫细菌Chlorobium tepidum的bchF和bchG同源物导入非硫细菌Rhodobacter capsulatus和蓝藻Synechocystis sp.PCC 6803中进行表达。新的色素积累在R.capsulatus和Synechocystis的细胞中。（2）光合机构的生物发生：铁硫簇组装因子IscR和IscS在C.tepidum中的高效表达和鉴定。铁氧还蛋白和铁硫型RC是从太阳细菌中分离出来的，它们在体外支持NADP^+的光还原。固氮酶样酶Dark POR是从荚膜红球藻中分离得到的一种酶，并对其生化性质进行了研究。（3）电子传递：胶状红卵藻的结合细胞色素为三血红素型，绿色芽绿球藻的结合细胞色素为四血红素型。构建了含有B.viridis细胞色素亚基的明胶红球菌嵌合RC，并研究了它们的电子传递。

项目成果

Overexpression of the ferredoxin gene located on the downstream of nif operon in the green sulfur bacterium Chlorobium tepidum

绿色硫细菌温绿球菌中nif操纵子下游铁氧还蛋白基因的过表达

• 发表时间: 2005
• 期刊: Photosynthesis : Fundamental Aspects to Global Perspectives (ed by A.van der Est, D.Bruce)
• 作者: Furusawa;T.;Seo;D.;Sakurai;H.;Inoue;K.
• 通讯作者: K.

Hirabayashi H., Ishi T., Takaichi S., Inoue K., Uehara: "The role of carotenoids in the photoadaptation of the brown-colored sulfur bacterium Chlorobium phaeobacteroides"Photochemistry and photobiology. 79(3)(掲載決定). (2004)

Hirabayashi H.、Ishi T.、Takaichi S.、Inoue K.、Uehara：“类胡萝卜素在棕色硫细菌 Chlorobium phaeobacteroides 的光适应中的作用”光化学和光生物学 79(3)（出版决定）。 ）

Minor but key chloirophylls in photosystem II.

光系统 II 中次要但关键的叶绿素。

• 发表时间: 2005
• 期刊: Photosyn.Res. 84
• 作者: Kobayashi M.;Watanabe S.Gotoh;Koizumi H.;Itoh Y.;Akiyama M.
• 通讯作者: Akiyama M.

Electron interaction between redox cofactors in photosynthetic reaction centers.

光合作用反应中心氧化还原辅助因子之间的电子相互作用。

• 发表时间: 2004
• 期刊: J.Biol.Chem. 279
• 作者: Alric J.;Cuni A;Maki H.;Nagashima K.V.P;Vermeglio A.;Rappaport F.
• 通讯作者: Rappaport F.

Identification of the primary electron donor in PSII of the Chl d-dominated cyanobacterium Acaryochloris mariana.

叶绿素 d 主导的蓝藻 Acaryocholis mariana 的 PSII 中初级电子供体的鉴定。

• 发表时间: 2004
• 期刊: FEBS Lett. 556
• 作者: Mimuro M.;Akimoto S.;Gotoh T.;Yokono M.;Akiyama M.;Tsuchiyia T.;Miyashita H.;Kobayashi M.;Yamazaki I.
• 通讯作者: Yamazaki I.